Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08685 and BPHYT_RS08690 are separated by 90 nucleotides BPHYT_RS08690 and BPHYT_RS08695 are separated by 157 nucleotides BPHYT_RS08695 and BPHYT_RS08700 are separated by 88 nucleotides
BPHYT_RS08685: BPHYT_RS08685 - hypothetical protein, at 1,974,732 to 1,974,956
_RS08685
BPHYT_RS08690: BPHYT_RS08690 - hypothetical protein, at 1,975,047 to 1,975,292
_RS08690
BPHYT_RS08695: BPHYT_RS08695 - tRNA-His, at 1,975,450 to 1,975,525
_RS08695
BPHYT_RS08700: BPHYT_RS08700 - tRNA-dihydrouridine synthase A, at 1,975,614 to 1,976,615
_RS08700
Position (kb) 1975
1976 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1974.507 kb on + strand at 1974.507 kb on + strand at 1974.519 kb on - strand at 1974.548 kb on + strand at 1974.677 kb on + strand at 1974.822 kb on - strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.925 kb on + strand, within BPHYT_RS08685 at 1974.926 kb on - strand, within BPHYT_RS08685 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.435 kb on - strand at 1975.698 kb on - strand at 1975.744 kb on + strand, within BPHYT_RS08700 at 1975.780 kb on + strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1976.036 kb on - strand, within BPHYT_RS08700 at 1976.170 kb on + strand, within BPHYT_RS08700 at 1976.171 kb on - strand, within BPHYT_RS08700 at 1976.347 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,974,507 + -0.2 1,974,507 + -0.1 1,974,519 - -0.6 1,974,548 + -0.4 1,974,677 + +0.0 1,974,822 - BPHYT_RS08685 0.40 -1.4 1,974,861 + BPHYT_RS08685 0.57 -0.9 1,974,861 + BPHYT_RS08685 0.57 -0.9 1,974,862 - BPHYT_RS08685 0.58 +0.3 1,974,862 - BPHYT_RS08685 0.58 +1.3 1,974,925 + BPHYT_RS08685 0.86 +0.8 1,974,926 - BPHYT_RS08685 0.86 +0.5 1,975,104 + BPHYT_RS08690 0.23 -0.6 1,975,104 + BPHYT_RS08690 0.23 -1.2 1,975,104 + BPHYT_RS08690 0.23 -1.7 1,975,104 + BPHYT_RS08690 0.23 -0.3 1,975,105 - BPHYT_RS08690 0.24 -2.7 1,975,105 - BPHYT_RS08690 0.24 -1.7 1,975,435 - +0.6 1,975,698 - +1.9 1,975,744 + BPHYT_RS08700 0.13 -0.1 1,975,780 + BPHYT_RS08700 0.17 -0.4 1,975,781 - BPHYT_RS08700 0.17 -1.5 1,975,781 - BPHYT_RS08700 0.17 -0.1 1,975,867 + BPHYT_RS08700 0.25 +0.9 1,975,867 + BPHYT_RS08700 0.25 +0.3 1,976,036 - BPHYT_RS08700 0.42 +0.5 1,976,170 + BPHYT_RS08700 0.55 -0.2 1,976,171 - BPHYT_RS08700 0.56 -0.8 1,976,347 + BPHYT_RS08700 0.73 +0.2 1,976,412 + BPHYT_RS08700 0.80 +1.0 1,976,412 + BPHYT_RS08700 0.80 -1.0
Or see this region's nucleotide sequence