Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07230 and BPHYT_RS07235 are separated by 109 nucleotides BPHYT_RS07235 and BPHYT_RS07240 overlap by 4 nucleotides BPHYT_RS07240 and BPHYT_RS07245 are separated by 11 nucleotides
BPHYT_RS07230: BPHYT_RS07230 - LysR family transcriptional regulator, at 1,642,065 to 1,643,042
_RS07230
BPHYT_RS07235: BPHYT_RS07235 - 2-hydroxymuconic semialdehyde dehydrogenase, at 1,643,152 to 1,644,642
_RS07235
BPHYT_RS07240: BPHYT_RS07240 - 2-keto-4-pentenoate hydratase, at 1,644,639 to 1,645,460
_RS07240
BPHYT_RS07245: BPHYT_RS07245 - acetaldehyde dehydrogenase, at 1,645,472 to 1,646,416
_RS07245
Position (kb) 1643
1644
1645 Strain fitness (log2 ratio) -1
0
1
2 at 1642.338 kb on - strand, within BPHYT_RS07230 at 1642.779 kb on - strand, within BPHYT_RS07230 at 1642.826 kb on + strand, within BPHYT_RS07230 at 1643.128 kb on + strand at 1643.129 kb on - strand at 1644.062 kb on - strand, within BPHYT_RS07235 at 1644.202 kb on + strand, within BPHYT_RS07235 at 1644.425 kb on - strand, within BPHYT_RS07235 at 1644.425 kb on - strand, within BPHYT_RS07235
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,642,338 - BPHYT_RS07230 0.28 -0.6 1,642,779 - BPHYT_RS07230 0.73 -0.1 1,642,826 + BPHYT_RS07230 0.78 +1.5 1,643,128 + -1.0 1,643,129 - +0.7 1,644,062 - BPHYT_RS07235 0.61 +0.2 1,644,202 + BPHYT_RS07235 0.70 +2.5 1,644,425 - BPHYT_RS07235 0.85 +0.5 1,644,425 - BPHYT_RS07235 0.85 -1.3
Or see this region's nucleotide sequence