Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05775

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05770 and BPHYT_RS05775 are separated by 81 nucleotidesBPHYT_RS05775 and BPHYT_RS05780 overlap by 4 nucleotidesBPHYT_RS05780 and BPHYT_RS05785 overlap by 4 nucleotidesBPHYT_RS05785 and BPHYT_RS05790 are separated by 62 nucleotides BPHYT_RS05770: BPHYT_RS05770 - peptidase U35, at 1,313,140 to 1,315,152 _RS05770 BPHYT_RS05775: BPHYT_RS05775 - hypothetical protein, at 1,315,234 to 1,315,563 _RS05775 BPHYT_RS05780: BPHYT_RS05780 - hypothetical protein, at 1,315,560 to 1,315,871 _RS05780 BPHYT_RS05785: BPHYT_RS05785 - hypothetical protein, at 1,315,868 to 1,316,299 _RS05785 BPHYT_RS05790: BPHYT_RS05790 - hypothetical protein, at 1,316,362 to 1,317,291 _RS05790 Position (kb) 1315 1316Strain fitness (log2 ratio) -3 -2 -1 0 1at 1314.346 kb on + strand, within BPHYT_RS05770at 1314.473 kb on - strand, within BPHYT_RS05770at 1314.478 kb on + strand, within BPHYT_RS05770at 1314.599 kb on - strand, within BPHYT_RS05770at 1314.640 kb on + strand, within BPHYT_RS05770at 1314.641 kb on - strand, within BPHYT_RS05770at 1314.641 kb on - strand, within BPHYT_RS05770at 1314.641 kb on - strand, within BPHYT_RS05770at 1314.739 kb on - strand, within BPHYT_RS05770at 1314.875 kb on - strand, within BPHYT_RS05770at 1314.875 kb on - strand, within BPHYT_RS05770at 1315.009 kb on + strandat 1315.040 kb on - strandat 1315.150 kb on + strandat 1315.150 kb on + strandat 1315.151 kb on - strandat 1315.376 kb on - strand, within BPHYT_RS05775at 1315.393 kb on + strand, within BPHYT_RS05775at 1315.722 kb on + strand, within BPHYT_RS05780at 1315.722 kb on + strand, within BPHYT_RS05780at 1315.735 kb on - strand, within BPHYT_RS05780

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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1,314,346 + BPHYT_RS05770 0.60 +0.3
1,314,473 - BPHYT_RS05770 0.66 -2.8
1,314,478 + BPHYT_RS05770 0.66 -0.1
1,314,599 - BPHYT_RS05770 0.72 +0.7
1,314,640 + BPHYT_RS05770 0.75 -0.9
1,314,641 - BPHYT_RS05770 0.75 +1.1
1,314,641 - BPHYT_RS05770 0.75 -0.4
1,314,641 - BPHYT_RS05770 0.75 -0.2
1,314,739 - BPHYT_RS05770 0.79 -0.0
1,314,875 - BPHYT_RS05770 0.86 -0.2
1,314,875 - BPHYT_RS05770 0.86 -0.6
1,315,009 + -1.3
1,315,040 - -1.8
1,315,150 + -0.5
1,315,150 + +1.3
1,315,151 - -0.6
1,315,376 - BPHYT_RS05775 0.43 -1.2
1,315,393 + BPHYT_RS05775 0.48 -2.0
1,315,722 + BPHYT_RS05780 0.52 -0.3
1,315,722 + BPHYT_RS05780 0.52 +0.2
1,315,735 - BPHYT_RS05780 0.56 +0.5

Or see this region's nucleotide sequence