Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04320 and BPHYT_RS04325 are separated by 197 nucleotides BPHYT_RS04325 and BPHYT_RS04330 are separated by 125 nucleotides
BPHYT_RS04320: BPHYT_RS04320 - hypothetical protein, at 994,426 to 995,727
_RS04320
BPHYT_RS04325: BPHYT_RS04325 - acyltransferase 3, at 995,925 to 997,088
_RS04325
BPHYT_RS04330: BPHYT_RS04330 - hypothetical protein, at 997,214 to 998,356
_RS04330
Position (kb) 995
996
997
998 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 995.003 kb on + strand, within BPHYT_RS04320 at 995.003 kb on + strand, within BPHYT_RS04320 at 995.253 kb on + strand, within BPHYT_RS04320 at 995.298 kb on + strand, within BPHYT_RS04320 at 995.299 kb on - strand, within BPHYT_RS04320 at 995.300 kb on + strand, within BPHYT_RS04320 at 995.300 kb on + strand, within BPHYT_RS04320 at 995.301 kb on - strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.344 kb on + strand, within BPHYT_RS04320 at 995.477 kb on - strand, within BPHYT_RS04320 at 995.634 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.765 kb on + strand at 995.766 kb on - strand at 995.924 kb on + strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.034 kb on - strand at 996.147 kb on - strand, within BPHYT_RS04325 at 996.150 kb on + strand, within BPHYT_RS04325 at 996.151 kb on - strand, within BPHYT_RS04325 at 996.151 kb on - strand, within BPHYT_RS04325 at 996.179 kb on + strand, within BPHYT_RS04325 at 996.180 kb on - strand, within BPHYT_RS04325 at 996.301 kb on - strand, within BPHYT_RS04325 at 996.363 kb on + strand, within BPHYT_RS04325 at 996.487 kb on + strand, within BPHYT_RS04325 at 996.509 kb on + strand, within BPHYT_RS04325 at 996.540 kb on - strand, within BPHYT_RS04325 at 996.705 kb on + strand, within BPHYT_RS04325 at 996.716 kb on + strand, within BPHYT_RS04325 at 996.873 kb on + strand, within BPHYT_RS04325 at 997.086 kb on + strand at 997.113 kb on + strand at 997.114 kb on - strand at 997.203 kb on + strand at 997.204 kb on - strand at 997.204 kb on - strand at 997.207 kb on + strand at 997.239 kb on - strand at 997.352 kb on + strand, within BPHYT_RS04330 at 997.352 kb on + strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.353 kb on - strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.360 kb on + strand, within BPHYT_RS04330 at 997.446 kb on - strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330 at 997.576 kb on + strand, within BPHYT_RS04330
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 995,003 + BPHYT_RS04320 0.44 +1.2 995,003 + BPHYT_RS04320 0.44 -1.2 995,253 + BPHYT_RS04320 0.64 -1.2 995,298 + BPHYT_RS04320 0.67 -2.9 995,299 - BPHYT_RS04320 0.67 -0.7 995,300 + BPHYT_RS04320 0.67 +0.3 995,300 + BPHYT_RS04320 0.67 +1.3 995,301 - BPHYT_RS04320 0.67 +2.9 995,344 + BPHYT_RS04320 0.71 +0.3 995,344 + BPHYT_RS04320 0.71 +0.2 995,344 + BPHYT_RS04320 0.71 -0.2 995,477 - BPHYT_RS04320 0.81 -1.7 995,634 + +0.3 995,765 + +0.8 995,765 + -0.1 995,765 + -0.5 995,765 + +0.3 995,766 - -4.1 995,924 + -2.4 996,034 - -2.3 996,034 - -1.5 996,034 - -1.4 996,034 - +0.7 996,147 - BPHYT_RS04325 0.19 -1.9 996,150 + BPHYT_RS04325 0.19 -0.4 996,151 - BPHYT_RS04325 0.19 -1.3 996,151 - BPHYT_RS04325 0.19 -2.9 996,179 + BPHYT_RS04325 0.22 +2.0 996,180 - BPHYT_RS04325 0.22 -1.2 996,301 - BPHYT_RS04325 0.32 -2.1 996,363 + BPHYT_RS04325 0.38 -1.5 996,487 + BPHYT_RS04325 0.48 -0.8 996,509 + BPHYT_RS04325 0.50 -2.8 996,540 - BPHYT_RS04325 0.53 -3.4 996,705 + BPHYT_RS04325 0.67 -0.1 996,716 + BPHYT_RS04325 0.68 +0.1 996,873 + BPHYT_RS04325 0.81 -1.3 997,086 + +0.3 997,113 + -2.1 997,114 - -0.9 997,203 + -2.2 997,204 - -2.5 997,204 - -2.7 997,207 + -0.5 997,239 - -2.1 997,352 + BPHYT_RS04330 0.12 -0.0 997,352 + BPHYT_RS04330 0.12 -1.4 997,353 - BPHYT_RS04330 0.12 -2.9 997,353 - BPHYT_RS04330 0.12 -1.0 997,360 + BPHYT_RS04330 0.13 -1.4 997,360 + BPHYT_RS04330 0.13 -2.5 997,360 + BPHYT_RS04330 0.13 +1.0 997,446 - BPHYT_RS04330 0.20 -2.9 997,576 + BPHYT_RS04330 0.32 -0.9 997,576 + BPHYT_RS04330 0.32 -1.2 997,576 + BPHYT_RS04330 0.32 -3.9
Or see this region's nucleotide sequence