Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03235 and BPHYT_RS03240 are separated by 140 nucleotides BPHYT_RS03240 and BPHYT_RS03245 are separated by 148 nucleotides BPHYT_RS03245 and BPHYT_RS03250 are separated by 238 nucleotides
BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182
_RS03235
BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162
_RS03240
BPHYT_RS03245: BPHYT_RS03245 - hypothetical protein, at 729,311 to 729,787
_RS03245
BPHYT_RS03250: BPHYT_RS03250 - diguanylate cyclase, at 730,026 to 733,136
_RS03250
Position (kb) 728
729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 727.423 kb on + strand at 727.493 kb on - strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 727.505 kb on + strand, within BPHYT_RS03235 at 728.205 kb on + strand at 728.346 kb on + strand at 728.347 kb on - strand at 728.360 kb on + strand at 728.361 kb on - strand at 728.493 kb on - strand, within BPHYT_RS03240 at 728.493 kb on - strand, within BPHYT_RS03240 at 729.165 kb on + strand at 729.166 kb on - strand at 729.166 kb on - strand at 729.166 kb on - strand at 729.249 kb on + strand at 729.258 kb on - strand at 729.258 kb on - strand at 729.306 kb on + strand at 729.306 kb on + strand at 729.306 kb on + strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.307 kb on - strand at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.374 kb on + strand, within BPHYT_RS03245 at 729.375 kb on - strand, within BPHYT_RS03245 at 729.625 kb on + strand, within BPHYT_RS03245 at 729.626 kb on - strand, within BPHYT_RS03245 at 729.705 kb on - strand, within BPHYT_RS03245 at 729.786 kb on - strand at 729.786 kb on - strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.789 kb on + strand at 729.820 kb on + strand at 729.820 kb on + strand at 729.821 kb on - strand at 729.824 kb on + strand at 729.859 kb on - strand at 729.896 kb on + strand at 729.943 kb on + strand at 730.107 kb on + strand at 730.107 kb on + strand at 730.108 kb on - strand at 730.109 kb on + strand at 730.110 kb on - strand at 730.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 727,423 + -0.7 727,493 - BPHYT_RS03235 0.11 -0.1 727,505 + BPHYT_RS03235 0.12 -2.1 727,505 + BPHYT_RS03235 0.12 -1.7 728,205 + +0.3 728,346 + -3.5 728,347 - +0.7 728,360 + -0.2 728,361 - +0.3 728,493 - BPHYT_RS03240 0.20 -0.9 728,493 - BPHYT_RS03240 0.20 +0.2 729,165 + +0.0 729,166 - -0.5 729,166 - -0.2 729,166 - -0.1 729,249 + +0.1 729,258 - -0.1 729,258 - +0.2 729,306 + +0.4 729,306 + +0.4 729,306 + -0.8 729,307 - -1.2 729,307 - -1.9 729,307 - +0.5 729,307 - -0.5 729,307 - -1.2 729,374 + BPHYT_RS03245 0.13 -1.0 729,374 + BPHYT_RS03245 0.13 -1.1 729,374 + BPHYT_RS03245 0.13 -0.9 729,374 + BPHYT_RS03245 0.13 -0.3 729,375 - BPHYT_RS03245 0.13 -0.8 729,625 + BPHYT_RS03245 0.66 -1.0 729,626 - BPHYT_RS03245 0.66 +0.3 729,705 - BPHYT_RS03245 0.83 -0.3 729,786 - -0.6 729,786 - -0.5 729,789 + +0.6 729,789 + +0.1 729,789 + -0.6 729,789 + -2.0 729,820 + +1.3 729,820 + +1.2 729,821 - -0.8 729,824 + -0.2 729,859 - -1.2 729,896 + -0.4 729,943 + +3.2 730,107 + -0.4 730,107 + +0.8 730,108 - +0.4 730,109 + -0.0 730,110 - -0.0 730,110 - +0.1
Or see this region's nucleotide sequence