Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01940 and BPHYT_RS01945 are separated by 79 nucleotides BPHYT_RS01945 and BPHYT_RS01950 are separated by 216 nucleotides BPHYT_RS01950 and BPHYT_RS01955 are separated by 26 nucleotides BPHYT_RS01955 and BPHYT_RS01960 overlap by 1 nucleotides
BPHYT_RS01940: BPHYT_RS01940 - 16S rRNA methyltransferase, at 440,739 to 441,620
_RS01940
BPHYT_RS01945: BPHYT_RS01945 - hypothetical protein, at 441,700 to 442,122
_RS01945
BPHYT_RS01950: BPHYT_RS01950 - phosphoheptose isomerase, at 442,339 to 442,926
_RS01950
BPHYT_RS01955: BPHYT_RS01955 - hypothetical protein, at 442,953 to 443,750
_RS01955
BPHYT_RS01960: BPHYT_RS01960 - cytochrome C, at 443,750 to 444,097
_RS01960
Position (kb) 442
443 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 441.343 kb on + strand, within BPHYT_RS01940 at 441.343 kb on + strand, within BPHYT_RS01940 at 441.344 kb on - strand, within BPHYT_RS01940 at 441.344 kb on - strand, within BPHYT_RS01940 at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.565 kb on + strand at 441.566 kb on - strand at 441.566 kb on - strand at 441.567 kb on + strand at 441.568 kb on - strand at 441.568 kb on - strand at 441.578 kb on + strand at 441.981 kb on + strand, within BPHYT_RS01945 at 441.981 kb on + strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 441.982 kb on - strand, within BPHYT_RS01945 at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.213 kb on + strand at 442.214 kb on - strand at 442.329 kb on + strand at 442.619 kb on - strand, within BPHYT_RS01950 at 442.799 kb on + strand, within BPHYT_RS01950 at 442.800 kb on - strand, within BPHYT_RS01950 at 442.800 kb on - strand, within BPHYT_RS01950 at 442.852 kb on - strand, within BPHYT_RS01950 at 442.947 kb on + strand at 442.947 kb on + strand at 442.948 kb on - strand at 442.948 kb on - strand at 442.961 kb on - strand at 442.961 kb on - strand at 443.356 kb on - strand, within BPHYT_RS01955 at 443.446 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955 at 443.542 kb on - strand, within BPHYT_RS01955
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 441,343 + BPHYT_RS01940 0.68 -1.2 441,343 + BPHYT_RS01940 0.68 -1.7 441,344 - BPHYT_RS01940 0.69 -0.8 441,344 - BPHYT_RS01940 0.69 +0.3 441,565 + -1.0 441,565 + +0.7 441,565 + -0.9 441,565 + -1.4 441,565 + -0.6 441,565 + +1.3 441,566 - +0.6 441,566 - -0.5 441,567 + -0.5 441,568 - +0.2 441,568 - -0.3 441,578 + +0.2 441,981 + BPHYT_RS01945 0.66 -2.2 441,981 + BPHYT_RS01945 0.66 +1.9 441,982 - BPHYT_RS01945 0.67 -2.0 441,982 - BPHYT_RS01945 0.67 -1.3 442,213 + -0.3 442,213 + -0.8 442,213 + +2.3 442,213 + -0.2 442,213 + -0.8 442,214 - -1.1 442,329 + +1.1 442,619 - BPHYT_RS01950 0.48 -2.1 442,799 + BPHYT_RS01950 0.78 -0.1 442,800 - BPHYT_RS01950 0.78 -0.1 442,800 - BPHYT_RS01950 0.78 -1.1 442,852 - BPHYT_RS01950 0.87 +0.5 442,947 + -3.2 442,947 + -0.9 442,948 - -3.1 442,948 - -1.9 442,961 - +0.4 442,961 - -1.1 443,356 - BPHYT_RS01955 0.51 -1.0 443,446 - BPHYT_RS01955 0.62 -2.0 443,542 - BPHYT_RS01955 0.74 +0.2 443,542 - BPHYT_RS01955 0.74 -1.9 443,542 - BPHYT_RS01955 0.74 +0.4
Or see this region's nucleotide sequence