Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01875 and BPHYT_RS01880 are separated by 252 nucleotides BPHYT_RS01880 and BPHYT_RS01885 are separated by 423 nucleotides
BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201
_RS01875
BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554
_RS01880
BPHYT_RS01885: BPHYT_RS01885 - branched-chain amino acid ABC transporter substrate-binding protein, at 428,978 to 430,123
_RS01885
Position (kb) 427
428
429 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 426.504 kb on + strand at 426.567 kb on + strand at 426.567 kb on + strand at 426.568 kb on - strand at 426.612 kb on + strand, within BPHYT_RS01875 at 426.613 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.772 kb on - strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.813 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 426.975 kb on + strand, within BPHYT_RS01875 at 427.070 kb on - strand, within BPHYT_RS01875 at 427.144 kb on - strand at 427.908 kb on + strand, within BPHYT_RS01880 at 427.909 kb on - strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.910 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.914 kb on + strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.915 kb on - strand, within BPHYT_RS01880 at 427.917 kb on + strand, within BPHYT_RS01880 at 427.918 kb on - strand, within BPHYT_RS01880 at 428.298 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.417 kb on + strand, within BPHYT_RS01880 at 428.418 kb on - strand, within BPHYT_RS01880 at 428.427 kb on + strand, within BPHYT_RS01880 at 428.621 kb on - strand at 428.636 kb on + strand at 428.749 kb on + strand at 428.749 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.752 kb on + strand at 428.803 kb on + strand at 428.804 kb on - strand at 428.804 kb on - strand at 428.819 kb on + strand at 428.944 kb on + strand at 428.945 kb on - strand at 428.975 kb on + strand at 428.975 kb on + strand at 428.975 kb on + strand at 428.975 kb on + strand at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.335 kb on + strand, within BPHYT_RS01885 at 429.336 kb on - strand at 429.336 kb on - strand, within BPHYT_RS01885 at 429.337 kb on + strand, within BPHYT_RS01885 at 429.461 kb on + strand, within BPHYT_RS01885 at 429.461 kb on + strand, within BPHYT_RS01885
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 426,504 + +0.5 426,567 + +1.1 426,567 + -0.2 426,568 - -0.7 426,612 + BPHYT_RS01875 0.16 -0.3 426,613 - BPHYT_RS01875 0.16 -2.3 426,772 - BPHYT_RS01875 0.38 +0.1 426,772 - BPHYT_RS01875 0.38 -1.4 426,813 + BPHYT_RS01875 0.44 -0.4 426,813 + BPHYT_RS01875 0.44 +0.3 426,975 + BPHYT_RS01875 0.68 +0.6 426,975 + BPHYT_RS01875 0.68 -1.1 427,070 - BPHYT_RS01875 0.81 -2.9 427,144 - +0.4 427,908 + BPHYT_RS01880 0.41 +0.4 427,909 - BPHYT_RS01880 0.41 -1.0 427,910 + BPHYT_RS01880 0.41 +0.3 427,910 + BPHYT_RS01880 0.41 +0.2 427,910 + BPHYT_RS01880 0.41 -0.6 427,910 + BPHYT_RS01880 0.41 -0.6 427,910 + BPHYT_RS01880 0.41 +0.9 427,914 + BPHYT_RS01880 0.42 +0.9 427,914 + BPHYT_RS01880 0.42 -1.0 427,914 + BPHYT_RS01880 0.42 -0.5 427,914 + BPHYT_RS01880 0.42 -0.7 427,914 + BPHYT_RS01880 0.42 +0.0 427,914 + BPHYT_RS01880 0.42 +0.2 427,914 + BPHYT_RS01880 0.42 +1.3 427,915 - BPHYT_RS01880 0.42 -0.5 427,915 - BPHYT_RS01880 0.42 +0.5 427,915 - BPHYT_RS01880 0.42 +0.0 427,917 + BPHYT_RS01880 0.42 -0.2 427,918 - BPHYT_RS01880 0.42 -0.6 428,298 + BPHYT_RS01880 0.77 +0.2 428,417 + BPHYT_RS01880 0.87 -0.5 428,417 + BPHYT_RS01880 0.87 +0.0 428,417 + BPHYT_RS01880 0.87 -0.9 428,417 + BPHYT_RS01880 0.87 -0.8 428,418 - BPHYT_RS01880 0.88 -0.5 428,427 + BPHYT_RS01880 0.88 -0.4 428,621 - -0.1 428,636 + -1.6 428,749 + +0.9 428,749 + -2.0 428,752 + +0.2 428,752 + +0.5 428,752 + +0.4 428,752 + +0.4 428,803 + +0.2 428,804 - -0.4 428,804 - +0.3 428,819 + -0.2 428,944 + +1.0 428,945 - +0.0 428,975 + -0.5 428,975 + +0.2 428,975 + -0.1 428,975 + +0.3 429,335 + BPHYT_RS01885 0.31 -0.7 429,335 + BPHYT_RS01885 0.31 -0.2 429,335 + BPHYT_RS01885 0.31 +0.6 429,335 + BPHYT_RS01885 0.31 +0.0 429,336 - +2.3 429,336 - BPHYT_RS01885 0.31 -0.3 429,337 + BPHYT_RS01885 0.31 -1.0 429,461 + BPHYT_RS01885 0.42 -0.5 429,461 + BPHYT_RS01885 0.42 -1.3
Or see this region's nucleotide sequence