Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01335 and BPHYT_RS01340 are separated by 428 nucleotides BPHYT_RS01340 and BPHYT_RS01345 are separated by 17 nucleotides BPHYT_RS01345 and BPHYT_RS01350 are separated by 107 nucleotides
BPHYT_RS01335: BPHYT_RS01335 - hypothetical protein, at 306,213 to 306,437
_RS01335
BPHYT_RS01340: BPHYT_RS01340 - ChrB domain-containing protein, at 306,866 to 307,429
_RS01340
BPHYT_RS01345: BPHYT_RS01345 - endonuclease/exonuclease/phosphatase, at 307,447 to 308,253
_RS01345
BPHYT_RS01350: BPHYT_RS01350 - MFS transporter permease, at 308,361 to 309,623
_RS01350
Position (kb) 306
307
308 Strain fitness (log2 ratio) -1
0
1
2
3 at 305.924 kb on + strand at 305.924 kb on + strand at 305.925 kb on - strand at 305.925 kb on - strand at 305.925 kb on - strand at 305.925 kb on - strand at 306.062 kb on + strand at 306.199 kb on + strand at 306.200 kb on - strand at 306.227 kb on + strand at 306.228 kb on - strand at 306.466 kb on + strand at 306.467 kb on - strand at 306.468 kb on + strand at 306.469 kb on - strand at 306.480 kb on + strand at 306.712 kb on + strand at 306.713 kb on - strand at 306.897 kb on - strand at 306.968 kb on + strand, within BPHYT_RS01340 at 307.223 kb on + strand, within BPHYT_RS01340 at 307.223 kb on + strand, within BPHYT_RS01340 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.367 kb on + strand, within BPHYT_RS01340 at 307.427 kb on + strand at 307.427 kb on + strand at 307.467 kb on + strand at 307.468 kb on - strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.471 kb on + strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.472 kb on - strand at 307.978 kb on + strand, within BPHYT_RS01345 at 307.978 kb on + strand, within BPHYT_RS01345 at 307.979 kb on - strand, within BPHYT_RS01345 at 308.137 kb on + strand, within BPHYT_RS01345 at 308.225 kb on - strand at 308.251 kb on + strand at 308.252 kb on - strand at 308.252 kb on - strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.296 kb on + strand at 308.297 kb on - strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.301 kb on + strand at 308.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 305,924 + -1.4 305,924 + -0.6 305,925 - +0.5 305,925 - +0.3 305,925 - -1.1 305,925 - -0.9 306,062 + -0.7 306,199 + -1.7 306,200 - +0.1 306,227 + -1.4 306,228 - -0.5 306,466 + +2.3 306,467 - -1.5 306,468 + +0.3 306,469 - +0.2 306,480 + +0.0 306,712 + -0.8 306,713 - -1.1 306,897 - -0.7 306,968 + BPHYT_RS01340 0.18 +0.9 307,223 + BPHYT_RS01340 0.63 -1.2 307,223 + BPHYT_RS01340 0.63 +1.3 307,367 + BPHYT_RS01340 0.89 -0.8 307,367 + BPHYT_RS01340 0.89 -0.8 307,427 + -1.1 307,427 + -1.2 307,467 + -0.8 307,468 - -0.7 307,471 + +0.4 307,471 + +0.0 307,471 + +0.2 307,472 - -0.0 307,472 - +0.1 307,472 - -0.1 307,978 + BPHYT_RS01345 0.66 +0.0 307,978 + BPHYT_RS01345 0.66 -0.7 307,979 - BPHYT_RS01345 0.66 -0.3 308,137 + BPHYT_RS01345 0.86 -0.4 308,225 - -0.7 308,251 + -0.4 308,252 - -1.6 308,252 - +0.0 308,296 + +3.3 308,296 + -1.2 308,296 + +0.6 308,297 - -0.4 308,301 + -1.2 308,301 + -0.1 308,301 + -0.9 308,301 + +2.3 308,302 - -0.3
Or see this region's nucleotide sequence