Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32520 and BPHYT_RS32525 are separated by 667 nucleotides BPHYT_RS32525 and BPHYT_RS32530 are separated by 250 nucleotides
BPHYT_RS32520: BPHYT_RS32520 - GntR family transcriptional regulator, at 2,867,705 to 2,868,553
_RS32520
BPHYT_RS32525: BPHYT_RS32525 - hypothetical protein, at 2,869,221 to 2,869,685
_RS32525
BPHYT_RS32530: BPHYT_RS32530 - transposase, at 2,869,936 to 2,871,492
_RS32530
Position (kb)
2869
2870 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2868.247 kb on + strand, within BPHYT_RS32520 at 2868.437 kb on + strand, within BPHYT_RS32520 at 2868.438 kb on - strand, within BPHYT_RS32520 at 2868.646 kb on - strand at 2868.651 kb on + strand at 2868.652 kb on - strand at 2869.140 kb on + strand at 2869.186 kb on + strand at 2869.206 kb on - strand at 2869.232 kb on + strand at 2869.233 kb on - strand at 2869.233 kb on - strand at 2869.340 kb on - strand, within BPHYT_RS32525 at 2869.408 kb on + strand, within BPHYT_RS32525 at 2869.641 kb on - strand at 2869.670 kb on - strand at 2869.670 kb on - strand at 2869.673 kb on + strand at 2869.866 kb on - strand at 2869.889 kb on + strand at 2869.900 kb on + strand at 2869.979 kb on + strand at 2870.030 kb on + strand at 2870.241 kb on - strand, within BPHYT_RS32530 at 2870.558 kb on + strand, within BPHYT_RS32530 at 2870.575 kb on + strand, within BPHYT_RS32530 at 2870.640 kb on - strand, within BPHYT_RS32530
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 2,868,247 + BPHYT_RS32520 0.64 -1.9 2,868,437 + BPHYT_RS32520 0.86 +0.3 2,868,438 - BPHYT_RS32520 0.86 -0.5 2,868,646 - +0.8 2,868,651 + +0.4 2,868,652 - -0.1 2,869,140 + +1.2 2,869,186 + -0.0 2,869,206 - +0.6 2,869,232 + +0.3 2,869,233 - -1.6 2,869,233 - +2.2 2,869,340 - BPHYT_RS32525 0.26 -1.3 2,869,408 + BPHYT_RS32525 0.40 -1.4 2,869,641 - -2.2 2,869,670 - +0.1 2,869,670 - +0.2 2,869,673 + +0.0 2,869,866 - -0.3 2,869,889 + +2.4 2,869,900 + +0.2 2,869,979 + +1.0 2,870,030 + +0.7 2,870,241 - BPHYT_RS32530 0.20 +0.7 2,870,558 + BPHYT_RS32530 0.40 -0.7 2,870,575 + BPHYT_RS32530 0.41 +1.1 2,870,640 - BPHYT_RS32530 0.45 -0.4
Or see this region's nucleotide sequence