Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26735 and BPHYT_RS26740 are separated by 28 nucleotides BPHYT_RS26740 and BPHYT_RS26745 are separated by 57 nucleotides BPHYT_RS26745 and BPHYT_RS26750 are separated by 4 nucleotides BPHYT_RS26750 and BPHYT_RS26755 are separated by 53 nucleotides
BPHYT_RS26735: BPHYT_RS26735 - FAD-dependent oxidoreductase, at 1,568,340 to 1,570,091
_RS26735
BPHYT_RS26740: BPHYT_RS26740 - hypothetical protein, at 1,570,120 to 1,570,311
_RS26740
BPHYT_RS26745: BPHYT_RS26745 - 2-dehydropantoate 2-reductase, at 1,570,369 to 1,571,352
_RS26745
BPHYT_RS26750: BPHYT_RS26750 - aldolase, at 1,571,357 to 1,572,148
_RS26750
BPHYT_RS26755: BPHYT_RS26755 - aromatic amino acid aminotransferase, at 1,572,202 to 1,573,401
_RS26755
Position (kb) 1570
1571
1572 Strain fitness (log2 ratio) -2
-1
0
1 at 1569.447 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.451 kb on - strand, within BPHYT_RS26735 at 1569.879 kb on - strand, within BPHYT_RS26735 at 1570.093 kb on - strand at 1570.098 kb on + strand at 1570.201 kb on + strand, within BPHYT_RS26740 at 1570.370 kb on + strand at 1571.080 kb on + strand, within BPHYT_RS26745 at 1572.005 kb on - strand, within BPHYT_RS26750 at 1572.015 kb on + strand, within BPHYT_RS26750 at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.243 kb on - strand at 1572.300 kb on - strand at 1572.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 1,569,447 - BPHYT_RS26735 0.63 -0.8 1,569,451 - BPHYT_RS26735 0.63 -0.6 1,569,451 - BPHYT_RS26735 0.63 -0.5 1,569,451 - BPHYT_RS26735 0.63 -0.1 1,569,879 - BPHYT_RS26735 0.88 -0.4 1,570,093 - -0.1 1,570,098 + +0.1 1,570,201 + BPHYT_RS26740 0.42 -1.4 1,570,370 + +1.1 1,571,080 + BPHYT_RS26745 0.72 +0.6 1,572,005 - BPHYT_RS26750 0.82 -0.0 1,572,015 + BPHYT_RS26750 0.83 -0.2 1,572,243 - -2.0 1,572,243 - -0.1 1,572,243 - +0.0 1,572,300 - -0.4 1,572,300 - -0.9
Or see this region's nucleotide sequence