Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10345 and BPHYT_RS10350 are separated by 23 nucleotides BPHYT_RS10350 and BPHYT_RS10355 are separated by 322 nucleotides BPHYT_RS10355 and BPHYT_RS10360 are separated by 132 nucleotides
BPHYT_RS10345: BPHYT_RS10345 - flagellar motor protein MotB, at 2,351,390 to 2,351,977
_RS10345
BPHYT_RS10350: BPHYT_RS10350 - hypothetical protein, at 2,352,001 to 2,352,468
_RS10350
BPHYT_RS10355: BPHYT_RS10355 - nitrate reductase, at 2,352,791 to 2,354,248
_RS10355
BPHYT_RS10360: BPHYT_RS10360 - MFS transporter, at 2,354,381 to 2,355,751
_RS10360
Position (kb)
2352
2353
2354
2355 Strain fitness (log2 ratio)
-1
0
1 at 2351.829 kb on + strand, within BPHYT_RS10345 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.183 kb on + strand, within BPHYT_RS10350 at 2352.303 kb on - strand, within BPHYT_RS10350 at 2352.560 kb on - strand at 2352.654 kb on - strand at 2352.821 kb on + strand at 2353.980 kb on - strand, within BPHYT_RS10355 at 2354.150 kb on - strand at 2354.153 kb on + strand at 2354.192 kb on + strand at 2354.361 kb on - strand at 2354.629 kb on - strand, within BPHYT_RS10360
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 2,351,829 + BPHYT_RS10345 0.75 -0.3 2,352,183 + BPHYT_RS10350 0.39 -0.1 2,352,183 + BPHYT_RS10350 0.39 +0.2 2,352,303 - BPHYT_RS10350 0.65 -0.8 2,352,560 - +0.3 2,352,654 - +0.3 2,352,821 + +1.4 2,353,980 - BPHYT_RS10355 0.82 -1.4 2,354,150 - -0.2 2,354,153 + -1.6 2,354,192 + +0.2 2,354,361 - -0.1 2,354,629 - BPHYT_RS10360 0.18 -0.4
Or see this region's nucleotide sequence