Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10155 and BPHYT_RS10160 are separated by 356 nucleotides BPHYT_RS10160 and BPHYT_RS10165 are separated by 513 nucleotides
BPHYT_RS10155: BPHYT_RS10155 - 2,2-dialkylglycine decarboxylase, at 2,311,270 to 2,312,571
_RS10155
BPHYT_RS10160: BPHYT_RS10160 - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 2,312,928 to 2,315,513
_RS10160
BPHYT_RS10165: BPHYT_RS10165 - transcriptional regulator, at 2,316,027 to 2,316,470
_RS10165
Position (kb)
2312
2313
2314
2315
2316 Strain fitness (log2 ratio)
-1
0
1
2 at 2311.942 kb on + strand, within BPHYT_RS10155 at 2312.162 kb on - strand, within BPHYT_RS10155 at 2312.170 kb on + strand, within BPHYT_RS10155 at 2312.172 kb on + strand, within BPHYT_RS10155 at 2312.173 kb on - strand, within BPHYT_RS10155 at 2312.442 kb on + strand at 2314.137 kb on + strand, within BPHYT_RS10160 at 2314.926 kb on + strand, within BPHYT_RS10160 at 2315.404 kb on - strand at 2315.511 kb on + strand at 2315.511 kb on + strand at 2315.512 kb on - strand at 2315.512 kb on - strand at 2315.640 kb on - strand at 2315.640 kb on - strand at 2315.660 kb on - strand at 2315.727 kb on + strand at 2315.728 kb on - strand at 2315.728 kb on - strand at 2316.232 kb on + strand, within BPHYT_RS10165 at 2316.276 kb on + strand, within BPHYT_RS10165
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 2,311,942 + BPHYT_RS10155 0.52 -1.4 2,312,162 - BPHYT_RS10155 0.69 -0.6 2,312,170 + BPHYT_RS10155 0.69 -0.1 2,312,172 + BPHYT_RS10155 0.69 -0.7 2,312,173 - BPHYT_RS10155 0.69 +1.9 2,312,442 + +0.0 2,314,137 + BPHYT_RS10160 0.47 -1.1 2,314,926 + BPHYT_RS10160 0.77 -0.4 2,315,404 - -0.6 2,315,511 + +0.7 2,315,511 + +1.9 2,315,512 - +0.2 2,315,512 - +0.5 2,315,640 - -0.4 2,315,640 - -0.5 2,315,660 - +1.0 2,315,727 + +0.1 2,315,728 - +0.1 2,315,728 - +0.7 2,316,232 + BPHYT_RS10165 0.46 -1.0 2,316,276 + BPHYT_RS10165 0.56 +1.1
Or see this region's nucleotide sequence