Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01060 and BPHYT_RS01065 are separated by 161 nucleotides BPHYT_RS01065 and BPHYT_RS01070 are separated by 48 nucleotides
BPHYT_RS01060: BPHYT_RS01060 - alcohol dehydrogenase, at 241,745 to 242,998
_RS01060
BPHYT_RS01065: BPHYT_RS01065 - GMC family oxidoreductase, at 243,160 to 244,938
_RS01065
BPHYT_RS01070: BPHYT_RS01070 - gluconate 2-dehydrogenase, at 244,987 to 245,706
_RS01070
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.367 kb on + strand, within BPHYT_RS01060 at 242.557 kb on + strand, within BPHYT_RS01060 at 242.602 kb on - strand, within BPHYT_RS01060 at 242.605 kb on + strand, within BPHYT_RS01060 at 242.862 kb on + strand, within BPHYT_RS01060 at 242.863 kb on - strand, within BPHYT_RS01060 at 242.885 kb on - strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.139 kb on + strand at 243.140 kb on - strand at 243.140 kb on - strand at 243.195 kb on - strand at 243.224 kb on + strand at 243.257 kb on + strand at 243.510 kb on - strand, within BPHYT_RS01065 at 243.602 kb on + strand, within BPHYT_RS01065 at 243.647 kb on + strand, within BPHYT_RS01065 at 243.648 kb on - strand, within BPHYT_RS01065 at 243.753 kb on - strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.872 kb on + strand, within BPHYT_RS01065 at 243.873 kb on - strand, within BPHYT_RS01065 at 243.947 kb on + strand, within BPHYT_RS01065 at 243.948 kb on - strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.058 kb on + strand, within BPHYT_RS01065 at 244.059 kb on - strand, within BPHYT_RS01065 at 244.059 kb on - strand, within BPHYT_RS01065 at 244.253 kb on + strand, within BPHYT_RS01065 at 244.514 kb on + strand, within BPHYT_RS01065 at 244.514 kb on + strand, within BPHYT_RS01065 at 244.670 kb on + strand, within BPHYT_RS01065 at 244.764 kb on - strand at 244.810 kb on - strand at 245.324 kb on + strand, within BPHYT_RS01070 at 245.325 kb on - strand, within BPHYT_RS01070 at 245.325 kb on - strand, within BPHYT_RS01070 at 245.342 kb on + strand, within BPHYT_RS01070 at 245.343 kb on - strand, within BPHYT_RS01070 at 245.420 kb on + strand, within BPHYT_RS01070 at 245.791 kb on + strand at 245.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 242,367 + BPHYT_RS01060 0.50 +0.8 242,367 + BPHYT_RS01060 0.50 +0.2 242,367 + BPHYT_RS01060 0.50 +0.8 242,557 + BPHYT_RS01060 0.65 +0.7 242,602 - BPHYT_RS01060 0.68 -0.1 242,605 + BPHYT_RS01060 0.69 -0.6 242,862 + BPHYT_RS01060 0.89 -1.1 242,863 - BPHYT_RS01060 0.89 +0.7 242,885 - +0.5 243,139 + -1.2 243,139 + -1.9 243,139 + +0.2 243,140 - -1.4 243,140 - -0.7 243,195 - -1.8 243,224 + +0.0 243,257 + -1.0 243,510 - BPHYT_RS01065 0.20 -1.6 243,602 + BPHYT_RS01065 0.25 -0.4 243,647 + BPHYT_RS01065 0.27 +0.0 243,648 - BPHYT_RS01065 0.27 -2.3 243,753 - BPHYT_RS01065 0.33 +1.4 243,872 + BPHYT_RS01065 0.40 +3.0 243,872 + BPHYT_RS01065 0.40 +0.1 243,872 + BPHYT_RS01065 0.40 +1.1 243,873 - BPHYT_RS01065 0.40 -1.1 243,947 + BPHYT_RS01065 0.44 -1.1 243,948 - BPHYT_RS01065 0.44 +1.4 244,058 + BPHYT_RS01065 0.50 +1.1 244,058 + BPHYT_RS01065 0.50 +0.1 244,058 + BPHYT_RS01065 0.50 +1.3 244,059 - BPHYT_RS01065 0.51 -0.4 244,059 - BPHYT_RS01065 0.51 -1.9 244,253 + BPHYT_RS01065 0.61 +3.1 244,514 + BPHYT_RS01065 0.76 +1.7 244,514 + BPHYT_RS01065 0.76 -0.2 244,670 + BPHYT_RS01065 0.85 +1.4 244,764 - -0.7 244,810 - -0.3 245,324 + BPHYT_RS01070 0.47 -0.2 245,325 - BPHYT_RS01070 0.47 +0.6 245,325 - BPHYT_RS01070 0.47 -1.3 245,342 + BPHYT_RS01070 0.49 +1.7 245,343 - BPHYT_RS01070 0.49 +0.9 245,420 + BPHYT_RS01070 0.60 -0.2 245,791 + -0.4 245,888 - -0.4
Or see this region's nucleotide sequence