Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30370 and BPHYT_RS30375 are separated by 52 nucleotides BPHYT_RS30375 and BPHYT_RS30380 overlap by 11 nucleotides BPHYT_RS30380 and BPHYT_RS30385 are separated by 48 nucleotides
BPHYT_RS30370: BPHYT_RS30370 - thioredoxin reductase, at 2,368,836 to 2,370,563
_RS30370
BPHYT_RS30375: BPHYT_RS30375 - hypothetical protein, at 2,370,616 to 2,372,166
_RS30375
BPHYT_RS30380: BPHYT_RS30380 - glyoxalase, at 2,372,156 to 2,373,106
_RS30380
BPHYT_RS30385: BPHYT_RS30385 - hypothetical protein, at 2,373,155 to 2,373,406
_RS30385
Position (kb)
2370
2371
2372
2373 Strain fitness (log2 ratio)
-2
-1
0
1 at 2369.776 kb on + strand, within BPHYT_RS30370 at 2370.569 kb on + strand at 2370.942 kb on + strand, within BPHYT_RS30375 at 2371.274 kb on + strand, within BPHYT_RS30375 at 2371.398 kb on - strand, within BPHYT_RS30375 at 2371.715 kb on + strand, within BPHYT_RS30375 at 2371.800 kb on + strand, within BPHYT_RS30375 at 2372.520 kb on + strand, within BPHYT_RS30380 at 2372.520 kb on + strand, within BPHYT_RS30380 at 2372.521 kb on - strand, within BPHYT_RS30380 at 2372.799 kb on + strand, within BPHYT_RS30380 at 2372.799 kb on + strand, within BPHYT_RS30380 at 2372.800 kb on - strand, within BPHYT_RS30380 at 2372.920 kb on - strand, within BPHYT_RS30380 at 2373.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 2,369,776 + BPHYT_RS30370 0.54 +1.0 2,370,569 + -0.5 2,370,942 + BPHYT_RS30375 0.21 -0.7 2,371,274 + BPHYT_RS30375 0.42 -0.7 2,371,398 - BPHYT_RS30375 0.50 -1.1 2,371,715 + BPHYT_RS30375 0.71 -1.0 2,371,800 + BPHYT_RS30375 0.76 -1.9 2,372,520 + BPHYT_RS30380 0.38 -0.2 2,372,520 + BPHYT_RS30380 0.38 +0.2 2,372,521 - BPHYT_RS30380 0.38 -1.2 2,372,799 + BPHYT_RS30380 0.68 +1.7 2,372,799 + BPHYT_RS30380 0.68 -0.5 2,372,800 - BPHYT_RS30380 0.68 -2.3 2,372,920 - BPHYT_RS30380 0.80 +0.1 2,373,113 - +1.3
Or see this region's nucleotide sequence