Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29005

Experiment: 24hr PHYTO MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28995 and BPHYT_RS29000 overlap by 4 nucleotidesBPHYT_RS29000 and BPHYT_RS29005 are separated by 21 nucleotidesBPHYT_RS29005 and BPHYT_RS29010 overlap by 4 nucleotides BPHYT_RS28995: BPHYT_RS28995 - histidine kinase, at 2,060,547 to 2,061,902 _RS28995 BPHYT_RS29000: BPHYT_RS29000 - transcriptional regulator, at 2,061,899 to 2,062,612 _RS29000 BPHYT_RS29005: BPHYT_RS29005 - transporter, at 2,062,634 to 2,065,765 _RS29005 BPHYT_RS29010: BPHYT_RS29010 - RND transporter, at 2,065,762 to 2,066,724 _RS29010 Position (kb) 2062 2063 2064 2065 2066Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2061.736 kb on + strand, within BPHYT_RS28995at 2061.737 kb on - strand, within BPHYT_RS28995at 2061.785 kb on - strandat 2061.888 kb on + strandat 2061.928 kb on - strandat 2063.094 kb on + strand, within BPHYT_RS29005at 2063.500 kb on - strand, within BPHYT_RS29005at 2063.695 kb on - strand, within BPHYT_RS29005at 2064.607 kb on - strand, within BPHYT_RS29005at 2064.962 kb on + strand, within BPHYT_RS29005at 2065.348 kb on - strand, within BPHYT_RS29005at 2065.663 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1
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2,061,736 + BPHYT_RS28995 0.88 +3.4
2,061,737 - BPHYT_RS28995 0.88 -0.2
2,061,785 - +0.4
2,061,888 + +0.1
2,061,928 - -0.3
2,063,094 + BPHYT_RS29005 0.15 +1.4
2,063,500 - BPHYT_RS29005 0.28 +0.4
2,063,695 - BPHYT_RS29005 0.34 -0.1
2,064,607 - BPHYT_RS29005 0.63 -0.4
2,064,962 + BPHYT_RS29005 0.74 +0.7
2,065,348 - BPHYT_RS29005 0.87 -2.0
2,065,663 - +0.6

Or see this region's nucleotide sequence