Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26290

Experiment: 24hr PHYTO MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26285 and BPHYT_RS26290 are separated by 265 nucleotidesBPHYT_RS26290 and BPHYT_RS26295 are separated by 207 nucleotidesBPHYT_RS26295 and BPHYT_RS26300 are separated by 220 nucleotides BPHYT_RS26285: BPHYT_RS26285 - glycogen debranching protein, at 1,467,580 to 1,469,796 _RS26285 BPHYT_RS26290: BPHYT_RS26290 - photosystem reaction center subunit H, at 1,470,062 to 1,470,469 _RS26290 BPHYT_RS26295: BPHYT_RS26295 - chemotaxis protein CheY, at 1,470,677 to 1,471,135 _RS26295 BPHYT_RS26300: BPHYT_RS26300 - ATP-dependent DNA ligase, at 1,471,356 to 1,473,032 _RS26300 Position (kb) 1470 1471Strain fitness (log2 ratio) -1 0 1at 1469.123 kb on + strand, within BPHYT_RS26285at 1469.615 kb on + strandat 1469.796 kb on + strandat 1469.797 kb on - strandat 1470.128 kb on + strand, within BPHYT_RS26290at 1470.256 kb on + strand, within BPHYT_RS26290at 1470.312 kb on - strand, within BPHYT_RS26290at 1471.290 kb on - strandat 1471.416 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1
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1,469,123 + BPHYT_RS26285 0.70 +0.3
1,469,615 + -1.6
1,469,796 + +0.0
1,469,797 - -0.3
1,470,128 + BPHYT_RS26290 0.16 -0.7
1,470,256 + BPHYT_RS26290 0.48 -0.7
1,470,312 - BPHYT_RS26290 0.61 -0.3
1,471,290 - +0.6
1,471,416 - +0.2

Or see this region's nucleotide sequence