Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22330 and BPHYT_RS22335 are separated by 266 nucleotides BPHYT_RS22335 and BPHYT_RS22340 are separated by 58 nucleotides
BPHYT_RS22325: BPHYT_RS22325 - histidine kinase, at 561,635 to 563,134
_RS22325
BPHYT_RS22330: BPHYT_RS22330 - PhoB family transcriptional regulator, at 563,128 to 563,865
_RS22330
BPHYT_RS22335: BPHYT_RS22335 - cytochrome C biogenesis protein, at 564,132 to 566,012
_RS22335
BPHYT_RS22340: BPHYT_RS22340 - methionine sulfoxide reductase A, at 566,071 to 566,802
_RS22340
Position (kb) 564
565
566
567 Strain fitness (log2 ratio) -1
0
1 at 563.134 kb on + strand at 563.256 kb on + strand, within BPHYT_RS22330 at 563.256 kb on + strand, within BPHYT_RS22330 at 563.257 kb on - strand, within BPHYT_RS22330 at 563.357 kb on + strand, within BPHYT_RS22330 at 563.981 kb on + strand at 564.022 kb on + strand at 564.110 kb on + strand at 564.136 kb on + strand at 564.137 kb on - strand at 564.137 kb on - strand at 564.137 kb on - strand at 564.843 kb on - strand, within BPHYT_RS22335 at 565.207 kb on - strand, within BPHYT_RS22335 at 565.336 kb on - strand, within BPHYT_RS22335 at 565.650 kb on + strand, within BPHYT_RS22335 at 566.041 kb on + strand at 566.042 kb on - strand at 566.359 kb on + strand, within BPHYT_RS22340 at 566.701 kb on + strand, within BPHYT_RS22340
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 563,134 + -1.3 563,256 + BPHYT_RS22330 0.17 +0.9 563,256 + BPHYT_RS22330 0.17 -0.9 563,257 - BPHYT_RS22330 0.17 +0.2 563,357 + BPHYT_RS22330 0.31 -1.4 563,981 + -0.5 564,022 + -0.7 564,110 + -0.2 564,136 + -0.6 564,137 - -1.2 564,137 - +0.0 564,137 - -1.0 564,843 - BPHYT_RS22335 0.38 +0.2 565,207 - BPHYT_RS22335 0.57 -0.0 565,336 - BPHYT_RS22335 0.64 -0.7 565,650 + BPHYT_RS22335 0.81 -0.1 566,041 + +0.7 566,042 - -0.6 566,359 + BPHYT_RS22340 0.39 -0.0 566,701 + BPHYT_RS22340 0.86 -0.2
Or see this region's nucleotide sequence