Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21615 and BPHYT_RS21620 overlap by 4 nucleotides BPHYT_RS21620 and BPHYT_RS21625 are separated by 117 nucleotides BPHYT_RS21625 and BPHYT_RS21630 are separated by 184 nucleotides
BPHYT_RS21615: BPHYT_RS21615 - FMN-dependent NADH-azoreductase, at 405,979 to 406,653
_RS21615
BPHYT_RS21620: BPHYT_RS21620 - GCN5 family acetyltransferase, at 406,650 to 407,117
_RS21620
BPHYT_RS21625: BPHYT_RS21625 - LysR family transcriptional regulator, at 407,235 to 408,149
_RS21625
BPHYT_RS21630: BPHYT_RS21630 - NmrA family transcriptional regulator, at 408,334 to 409,197
_RS21630
Position (kb) 407
408
409 Strain fitness (log2 ratio) -2
-1
0
1 at 406.735 kb on + strand, within BPHYT_RS21620 at 407.320 kb on + strand at 407.321 kb on - strand at 407.623 kb on + strand, within BPHYT_RS21625 at 408.109 kb on - strand at 408.215 kb on - strand at 408.308 kb on - strand at 408.308 kb on - strand at 408.311 kb on + strand at 408.312 kb on - strand at 408.315 kb on + strand at 408.315 kb on + strand at 409.053 kb on + strand, within BPHYT_RS21630
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 406,735 + BPHYT_RS21620 0.18 -0.9 407,320 + -1.2 407,321 - -1.8 407,623 + BPHYT_RS21625 0.42 -1.3 408,109 - -0.5 408,215 - -0.3 408,308 - -1.4 408,308 - -0.3 408,311 + -0.6 408,312 - +0.2 408,315 + -1.0 408,315 + +0.1 409,053 + BPHYT_RS21630 0.83 -0.1
Or see this region's nucleotide sequence