Experiment: 24hr PHYTO MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09790 and BPHYT_RS09795 are separated by 710 nucleotides BPHYT_RS09795 and BPHYT_RS09800 are separated by 361 nucleotides BPHYT_RS09800 and BPHYT_RS09805 are separated by 251 nucleotides
BPHYT_RS09790: BPHYT_RS09790 - acyltransferase, at 2,224,009 to 2,225,220
_RS09790
BPHYT_RS09795: BPHYT_RS09795 - UTP--glucose-1-phosphate uridylyltransferase, at 2,225,931 to 2,226,848
_RS09795
BPHYT_RS09800: BPHYT_RS09800 - hypothetical protein, at 2,227,210 to 2,227,542
_RS09800
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2224.970 kb on + strand, within BPHYT_RS09790 at 2225.060 kb on + strand, within BPHYT_RS09790 at 2225.061 kb on - strand, within BPHYT_RS09790 at 2225.312 kb on + strand at 2225.313 kb on - strand at 2225.313 kb on - strand at 2225.316 kb on - strand at 2225.622 kb on - strand at 2225.832 kb on - strand at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.234 kb on + strand, within BPHYT_RS09795 at 2226.235 kb on - strand, within BPHYT_RS09795 at 2226.553 kb on - strand, within BPHYT_RS09795 at 2226.789 kb on + strand at 2226.790 kb on - strand at 2226.790 kb on - strand at 2226.790 kb on - strand at 2226.791 kb on + strand at 2226.930 kb on + strand at 2227.030 kb on + strand at 2227.227 kb on - strand at 2227.347 kb on + strand, within BPHYT_RS09800 at 2227.541 kb on + strand at 2227.541 kb on + strand at 2227.624 kb on + strand at 2227.668 kb on + strand at 2227.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO MOI 0.1 remove 2,224,970 + BPHYT_RS09790 0.79 +2.3 2,225,060 + BPHYT_RS09790 0.87 -0.1 2,225,061 - BPHYT_RS09790 0.87 +0.3 2,225,312 + +0.0 2,225,313 - +0.0 2,225,313 - +0.8 2,225,316 - -0.8 2,225,622 - +0.0 2,225,832 - -0.3 2,226,234 + BPHYT_RS09795 0.33 -1.1 2,226,234 + BPHYT_RS09795 0.33 -2.1 2,226,234 + BPHYT_RS09795 0.33 +0.2 2,226,235 - BPHYT_RS09795 0.33 -0.1 2,226,553 - BPHYT_RS09795 0.68 +0.4 2,226,789 + +0.3 2,226,790 - +0.9 2,226,790 - -1.2 2,226,790 - +0.2 2,226,791 + -0.4 2,226,930 + -0.4 2,227,030 + +0.0 2,227,227 - +0.4 2,227,347 + BPHYT_RS09800 0.41 -0.1 2,227,541 + +0.3 2,227,541 + +0.6 2,227,624 + -0.1 2,227,668 + +1.8 2,227,789 + -0.8
Or see this region's nucleotide sequence