Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32950 and BPHYT_RS32955 are separated by 468 nucleotides BPHYT_RS32955 and BPHYT_RS32960 are separated by 13 nucleotides BPHYT_RS32960 and BPHYT_RS32965 are separated by 92 nucleotides
BPHYT_RS32950: BPHYT_RS32950 - transposase, at 2,971,779 to 2,972,948
_RS32950
BPHYT_RS32955: BPHYT_RS32955 - polyketide synthase, at 2,973,417 to 2,974,073
_RS32955
BPHYT_RS32960: BPHYT_RS32960 - short-chain dehydrogenase, at 2,974,087 to 2,974,812
_RS32960
BPHYT_RS32965: BPHYT_RS32965 - HxlR family transcriptional regulator, at 2,974,905 to 2,975,300
_RS32965
Position (kb)
2973
2974
2975 Strain fitness (log2 ratio)
-1
0
1 at 2973.291 kb on + strand at 2973.355 kb on + strand at 2973.383 kb on + strand at 2973.383 kb on + strand at 2973.383 kb on + strand at 2973.384 kb on - strand at 2973.418 kb on + strand at 2973.419 kb on - strand at 2973.419 kb on - strand at 2973.429 kb on + strand at 2973.491 kb on + strand, within BPHYT_RS32955 at 2973.703 kb on + strand, within BPHYT_RS32955 at 2973.741 kb on - strand, within BPHYT_RS32955 at 2973.822 kb on + strand, within BPHYT_RS32955 at 2973.914 kb on - strand, within BPHYT_RS32955 at 2973.914 kb on - strand, within BPHYT_RS32955 at 2974.029 kb on - strand at 2974.053 kb on - strand at 2974.073 kb on + strand at 2974.074 kb on - strand at 2974.320 kb on - strand, within BPHYT_RS32960 at 2974.320 kb on - strand, within BPHYT_RS32960 at 2974.833 kb on + strand at 2974.834 kb on - strand at 2974.852 kb on - strand at 2974.852 kb on - strand at 2974.861 kb on - strand at 2974.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,973,291 + +0.3 2,973,355 + -0.3 2,973,383 + +0.2 2,973,383 + -0.1 2,973,383 + -0.5 2,973,384 - -1.0 2,973,418 + +0.1 2,973,419 - -0.0 2,973,419 - -0.4 2,973,429 + -0.7 2,973,491 + BPHYT_RS32955 0.11 -1.4 2,973,703 + BPHYT_RS32955 0.44 +0.1 2,973,741 - BPHYT_RS32955 0.49 -0.2 2,973,822 + BPHYT_RS32955 0.62 +0.8 2,973,914 - BPHYT_RS32955 0.76 +0.1 2,973,914 - BPHYT_RS32955 0.76 -0.3 2,974,029 - +0.3 2,974,053 - -0.0 2,974,073 + -0.6 2,974,074 - -0.5 2,974,320 - BPHYT_RS32960 0.32 +1.2 2,974,320 - BPHYT_RS32960 0.32 +0.2 2,974,833 + +0.2 2,974,834 - -0.5 2,974,852 - +0.3 2,974,852 - +0.2 2,974,861 - +0.7 2,974,886 - -0.7
Or see this region's nucleotide sequence