Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32465 and BPHYT_RS32470 are separated by 3 nucleotides BPHYT_RS32470 and BPHYT_RS32475 are separated by 18 nucleotides BPHYT_RS32475 and BPHYT_RS32480 are separated by 292 nucleotides BPHYT_RS32480 and BPHYT_RS32485 are separated by 65 nucleotides
BPHYT_RS32465: BPHYT_RS32465 - membrane protein, at 2,856,612 to 2,857,667
_RS32465
BPHYT_RS32470: BPHYT_RS32470 - membrane protein, at 2,857,671 to 2,857,805
_RS32470
BPHYT_RS32475: BPHYT_RS32475 - dihydroxyacetone kinase, at 2,857,824 to 2,859,527
_RS32475
BPHYT_RS32480: BPHYT_RS32480 - tRNA-Val, at 2,859,820 to 2,859,896
_RS32480
BPHYT_RS32485: BPHYT_RS32485 - microcystin LR degradation protein MlrC, at 2,859,962 to 2,861,452
_RS32485
Position (kb)
2857
2858
2859
2860 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2857.323 kb on + strand, within BPHYT_RS32465 at 2857.323 kb on + strand, within BPHYT_RS32465 at 2857.324 kb on - strand, within BPHYT_RS32465 at 2857.550 kb on - strand, within BPHYT_RS32465 at 2857.680 kb on - strand at 2857.725 kb on + strand, within BPHYT_RS32470 at 2857.773 kb on - strand, within BPHYT_RS32470 at 2857.897 kb on - strand at 2857.915 kb on + strand at 2859.057 kb on + strand, within BPHYT_RS32475 at 2859.058 kb on - strand, within BPHYT_RS32475 at 2859.058 kb on - strand, within BPHYT_RS32475 at 2859.203 kb on - strand, within BPHYT_RS32475 at 2859.544 kb on + strand at 2859.545 kb on - strand at 2859.545 kb on - strand at 2859.583 kb on - strand at 2859.607 kb on + strand at 2859.607 kb on + strand at 2859.608 kb on - strand at 2859.608 kb on - strand at 2859.614 kb on - strand at 2859.709 kb on - strand at 2859.749 kb on - strand at 2860.116 kb on + strand, within BPHYT_RS32485 at 2860.116 kb on + strand, within BPHYT_RS32485 at 2860.117 kb on - strand, within BPHYT_RS32485 at 2860.281 kb on + strand, within BPHYT_RS32485
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,857,323 + BPHYT_RS32465 0.67 -0.1 2,857,323 + BPHYT_RS32465 0.67 +3.3 2,857,324 - BPHYT_RS32465 0.67 -0.6 2,857,550 - BPHYT_RS32465 0.89 +0.7 2,857,680 - +0.2 2,857,725 + BPHYT_RS32470 0.40 -0.6 2,857,773 - BPHYT_RS32470 0.76 -0.7 2,857,897 - -0.4 2,857,915 + -0.0 2,859,057 + BPHYT_RS32475 0.72 -0.9 2,859,058 - BPHYT_RS32475 0.72 +1.4 2,859,058 - BPHYT_RS32475 0.72 +1.2 2,859,203 - BPHYT_RS32475 0.81 -0.4 2,859,544 + -0.3 2,859,545 - -0.8 2,859,545 - -0.1 2,859,583 - -2.6 2,859,607 + +0.0 2,859,607 + +0.0 2,859,608 - -0.3 2,859,608 - -0.4 2,859,614 - +0.1 2,859,709 - -1.7 2,859,749 - -2.0 2,860,116 + BPHYT_RS32485 0.10 -1.2 2,860,116 + BPHYT_RS32485 0.10 -1.4 2,860,117 - BPHYT_RS32485 0.10 -0.2 2,860,281 + BPHYT_RS32485 0.21 +1.6
Or see this region's nucleotide sequence