Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32475

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32465 and BPHYT_RS32470 are separated by 3 nucleotidesBPHYT_RS32470 and BPHYT_RS32475 are separated by 18 nucleotidesBPHYT_RS32475 and BPHYT_RS32480 are separated by 292 nucleotidesBPHYT_RS32480 and BPHYT_RS32485 are separated by 65 nucleotides BPHYT_RS32465: BPHYT_RS32465 - membrane protein, at 2,856,612 to 2,857,667 _RS32465 BPHYT_RS32470: BPHYT_RS32470 - membrane protein, at 2,857,671 to 2,857,805 _RS32470 BPHYT_RS32475: BPHYT_RS32475 - dihydroxyacetone kinase, at 2,857,824 to 2,859,527 _RS32475 BPHYT_RS32480: BPHYT_RS32480 - tRNA-Val, at 2,859,820 to 2,859,896 _RS32480 BPHYT_RS32485: BPHYT_RS32485 - microcystin LR degradation protein MlrC, at 2,859,962 to 2,861,452 _RS32485 Position (kb) 2857 2858 2859 2860Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2857.323 kb on + strand, within BPHYT_RS32465at 2857.323 kb on + strand, within BPHYT_RS32465at 2857.324 kb on - strand, within BPHYT_RS32465at 2857.550 kb on - strand, within BPHYT_RS32465at 2857.680 kb on - strandat 2857.725 kb on + strand, within BPHYT_RS32470at 2857.773 kb on - strand, within BPHYT_RS32470at 2857.897 kb on - strandat 2857.915 kb on + strandat 2859.057 kb on + strand, within BPHYT_RS32475at 2859.058 kb on - strand, within BPHYT_RS32475at 2859.058 kb on - strand, within BPHYT_RS32475at 2859.203 kb on - strand, within BPHYT_RS32475at 2859.544 kb on + strandat 2859.545 kb on - strandat 2859.545 kb on - strandat 2859.583 kb on - strandat 2859.607 kb on + strandat 2859.607 kb on + strandat 2859.608 kb on - strandat 2859.608 kb on - strandat 2859.614 kb on - strandat 2859.709 kb on - strandat 2859.749 kb on - strandat 2860.116 kb on + strand, within BPHYT_RS32485at 2860.116 kb on + strand, within BPHYT_RS32485at 2860.117 kb on - strand, within BPHYT_RS32485at 2860.281 kb on + strand, within BPHYT_RS32485

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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2,857,323 + BPHYT_RS32465 0.67 -0.1
2,857,323 + BPHYT_RS32465 0.67 +3.3
2,857,324 - BPHYT_RS32465 0.67 -0.6
2,857,550 - BPHYT_RS32465 0.89 +0.7
2,857,680 - +0.2
2,857,725 + BPHYT_RS32470 0.40 -0.6
2,857,773 - BPHYT_RS32470 0.76 -0.7
2,857,897 - -0.4
2,857,915 + -0.0
2,859,057 + BPHYT_RS32475 0.72 -0.9
2,859,058 - BPHYT_RS32475 0.72 +1.4
2,859,058 - BPHYT_RS32475 0.72 +1.2
2,859,203 - BPHYT_RS32475 0.81 -0.4
2,859,544 + -0.3
2,859,545 - -0.8
2,859,545 - -0.1
2,859,583 - -2.6
2,859,607 + +0.0
2,859,607 + +0.0
2,859,608 - -0.3
2,859,608 - -0.4
2,859,614 - +0.1
2,859,709 - -1.7
2,859,749 - -2.0
2,860,116 + BPHYT_RS32485 0.10 -1.2
2,860,116 + BPHYT_RS32485 0.10 -1.4
2,860,117 - BPHYT_RS32485 0.10 -0.2
2,860,281 + BPHYT_RS32485 0.21 +1.6

Or see this region's nucleotide sequence