Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32285 and BPHYT_RS32290 are separated by 106 nucleotides BPHYT_RS32290 and BPHYT_RS32295 are separated by 86 nucleotides BPHYT_RS32295 and BPHYT_RS32300 are separated by 6 nucleotides
BPHYT_RS32285: BPHYT_RS32285 - aromatic amino acid aminotransferase, at 2,813,224 to 2,814,414
_RS32285
BPHYT_RS32290: BPHYT_RS32290 - hypothetical protein, at 2,814,521 to 2,815,210
_RS32290
BPHYT_RS32295: BPHYT_RS32295 - ATP-binding protein, at 2,815,297 to 2,816,085
_RS32295
BPHYT_RS32300: BPHYT_RS32300 - integrase, at 2,816,092 to 2,817,609
_RS32300
Position (kb)
2814
2815
2816 Strain fitness (log2 ratio)
-2
-1
0
1 at 2813.969 kb on + strand, within BPHYT_RS32285 at 2813.970 kb on - strand, within BPHYT_RS32285 at 2814.071 kb on - strand, within BPHYT_RS32285 at 2814.118 kb on - strand, within BPHYT_RS32285 at 2814.193 kb on - strand, within BPHYT_RS32285 at 2814.198 kb on + strand, within BPHYT_RS32285 at 2814.199 kb on - strand, within BPHYT_RS32285 at 2814.308 kb on + strand at 2814.535 kb on - strand at 2814.846 kb on + strand, within BPHYT_RS32290 at 2814.857 kb on + strand, within BPHYT_RS32290 at 2815.094 kb on + strand, within BPHYT_RS32290
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,813,969 + BPHYT_RS32285 0.63 +0.1 2,813,970 - BPHYT_RS32285 0.63 -0.7 2,814,071 - BPHYT_RS32285 0.71 -1.0 2,814,118 - BPHYT_RS32285 0.75 -0.4 2,814,193 - BPHYT_RS32285 0.81 -1.6 2,814,198 + BPHYT_RS32285 0.82 +0.5 2,814,199 - BPHYT_RS32285 0.82 -0.5 2,814,308 + +0.1 2,814,535 - -0.5 2,814,846 + BPHYT_RS32290 0.47 -0.4 2,814,857 + BPHYT_RS32290 0.49 -1.0 2,815,094 + BPHYT_RS32290 0.83 -2.4
Or see this region's nucleotide sequence