Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32205

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32195 and BPHYT_RS32200 are separated by 41 nucleotidesBPHYT_RS32200 and BPHYT_RS32205 are separated by 290 nucleotidesBPHYT_RS32205 and BPHYT_RS32210 are separated by 377 nucleotidesBPHYT_RS32210 and BPHYT_RS32215 overlap by 8 nucleotides BPHYT_RS32195: BPHYT_RS32195 - type VI secretion protein, at 2,796,065 to 2,796,406 _RS32195 BPHYT_RS32200: BPHYT_RS32200 - lytic transglycosylase, at 2,796,448 to 2,797,092 _RS32200 BPHYT_RS32205: BPHYT_RS32205 - hypothetical protein, at 2,797,383 to 2,797,622 _RS32205 BPHYT_RS32210: BPHYT_RS32210 - hypothetical protein, at 2,798,000 to 2,798,284 _RS32210 BPHYT_RS32215: BPHYT_RS32215 - AlpA family transcriptional regulator, at 2,798,277 to 2,798,510 _RS32215 Position (kb) 2797 2798Strain fitness (log2 ratio) -2 -1 0 1at 2796.601 kb on + strand, within BPHYT_RS32200at 2796.657 kb on - strand, within BPHYT_RS32200at 2797.960 kb on - strandat 2798.141 kb on + strand, within BPHYT_RS32210

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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2,796,601 + BPHYT_RS32200 0.24 -1.1
2,796,657 - BPHYT_RS32200 0.32 +1.4
2,797,960 - +0.4
2,798,141 + BPHYT_RS32210 0.49 -2.5

Or see this region's nucleotide sequence