Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS32185 and BPHYT_RS32190 are separated by 1 nucleotides BPHYT_RS32190 and BPHYT_RS32195 are separated by 16 nucleotides BPHYT_RS32195 and BPHYT_RS32200 are separated by 41 nucleotides BPHYT_RS32200 and BPHYT_RS32205 are separated by 290 nucleotides
BPHYT_RS32185: BPHYT_RS32185 - type IV secretion protein VirB4, at 2,793,237 to 2,795,702
_RS32185
BPHYT_RS32190: BPHYT_RS32190 - conjugal transfer protein TraD, at 2,795,704 to 2,796,048
_RS32190
BPHYT_RS32195: BPHYT_RS32195 - type VI secretion protein, at 2,796,065 to 2,796,406
_RS32195
BPHYT_RS32200: BPHYT_RS32200 - lytic transglycosylase, at 2,796,448 to 2,797,092
_RS32200
BPHYT_RS32205: BPHYT_RS32205 - hypothetical protein, at 2,797,383 to 2,797,622
_RS32205
Position (kb)
2796
2797 Strain fitness (log2 ratio)
-1
0
1 at 2795.180 kb on - strand, within BPHYT_RS32185 at 2795.223 kb on + strand, within BPHYT_RS32185 at 2795.335 kb on + strand, within BPHYT_RS32185 at 2795.336 kb on - strand, within BPHYT_RS32185 at 2795.587 kb on + strand at 2795.798 kb on + strand, within BPHYT_RS32190 at 2796.021 kb on + strand at 2796.025 kb on - strand at 2796.025 kb on - strand at 2796.601 kb on + strand, within BPHYT_RS32200 at 2796.657 kb on - strand, within BPHYT_RS32200
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,795,180 - BPHYT_RS32185 0.79 -0.2 2,795,223 + BPHYT_RS32185 0.81 -0.0 2,795,335 + BPHYT_RS32185 0.85 +0.2 2,795,336 - BPHYT_RS32185 0.85 -0.4 2,795,587 + +0.2 2,795,798 + BPHYT_RS32190 0.27 -1.0 2,796,021 + -1.2 2,796,025 - -0.5 2,796,025 - -0.3 2,796,601 + BPHYT_RS32200 0.24 -1.1 2,796,657 - BPHYT_RS32200 0.32 +1.4
Or see this region's nucleotide sequence