Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27725 and BPHYT_RS27730 are separated by 46 nucleotides BPHYT_RS27730 and BPHYT_RS27735 are separated by 171 nucleotides BPHYT_RS27735 and BPHYT_RS27740 are separated by 37 nucleotides BPHYT_RS27740 and BPHYT_RS27745 are separated by 297 nucleotides
BPHYT_RS27725: BPHYT_RS27725 - hypothetical protein, at 1,783,832 to 1,784,050
_RS27725
BPHYT_RS27730: BPHYT_RS27730 - LuxR family transcriptional regulator, at 1,784,097 to 1,784,744
_RS27730
BPHYT_RS27735: BPHYT_RS27735 - nitroreductase, at 1,784,916 to 1,785,947
_RS27735
BPHYT_RS27740: BPHYT_RS27740 - hypothetical protein, at 1,785,985 to 1,786,260
_RS27740
BPHYT_RS27745: BPHYT_RS27745 - nitroreductase, at 1,786,558 to 1,787,229
_RS27745
Position (kb)
1784
1785
1786 Strain fitness (log2 ratio)
-1
0
1
2 at 1783.943 kb on - strand, within BPHYT_RS27725 at 1784.799 kb on + strand at 1784.888 kb on + strand at 1785.204 kb on + strand, within BPHYT_RS27735 at 1785.205 kb on - strand, within BPHYT_RS27735 at 1785.616 kb on + strand, within BPHYT_RS27735 at 1785.663 kb on - strand, within BPHYT_RS27735 at 1785.663 kb on - strand, within BPHYT_RS27735 at 1785.937 kb on + strand at 1785.938 kb on - strand at 1786.520 kb on + strand at 1786.520 kb on + strand at 1786.647 kb on - strand, within BPHYT_RS27745 at 1786.844 kb on - strand, within BPHYT_RS27745
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 1,783,943 - BPHYT_RS27725 0.51 +2.3 1,784,799 + -0.4 1,784,888 + -0.8 1,785,204 + BPHYT_RS27735 0.28 +0.6 1,785,205 - BPHYT_RS27735 0.28 -1.0 1,785,616 + BPHYT_RS27735 0.68 -0.0 1,785,663 - BPHYT_RS27735 0.72 +1.6 1,785,663 - BPHYT_RS27735 0.72 -0.3 1,785,937 + +0.0 1,785,938 - -0.6 1,786,520 + +1.7 1,786,520 + +0.6 1,786,647 - BPHYT_RS27745 0.13 -0.9 1,786,844 - BPHYT_RS27745 0.43 -0.8
Or see this region's nucleotide sequence