Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26625

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26620 and BPHYT_RS26625 are separated by 66 nucleotidesBPHYT_RS26625 and BPHYT_RS26630 are separated by 2 nucleotides BPHYT_RS26620: BPHYT_RS26620 - MFS transporter, at 1,539,683 to 1,541,176 _RS26620 BPHYT_RS26625: BPHYT_RS26625 - lipoprotein signal peptide, at 1,541,243 to 1,542,367 _RS26625 BPHYT_RS26630: BPHYT_RS26630 - AraC family transcriptional regulator, at 1,542,370 to 1,543,260 _RS26630 Position (kb) 1541 1542 1543Strain fitness (log2 ratio) -1 0 1at 1540.538 kb on + strand, within BPHYT_RS26620at 1540.539 kb on - strand, within BPHYT_RS26620at 1541.236 kb on - strandat 1541.236 kb on - strandat 1541.792 kb on - strand, within BPHYT_RS26625at 1542.252 kb on - strand, within BPHYT_RS26625at 1542.537 kb on - strand, within BPHYT_RS26630at 1542.998 kb on + strand, within BPHYT_RS26630at 1543.270 kb on - strandat 1543.344 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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1,540,538 + BPHYT_RS26620 0.57 -0.4
1,540,539 - BPHYT_RS26620 0.57 -1.3
1,541,236 - -1.4
1,541,236 - -0.2
1,541,792 - BPHYT_RS26625 0.49 -0.0
1,542,252 - BPHYT_RS26625 0.90 +0.9
1,542,537 - BPHYT_RS26630 0.19 -0.7
1,542,998 + BPHYT_RS26630 0.70 +0.6
1,543,270 - -1.1
1,543,344 + +0.1

Or see this region's nucleotide sequence