Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS26300

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS26290 and BPHYT_RS26295 are separated by 207 nucleotidesBPHYT_RS26295 and BPHYT_RS26300 are separated by 220 nucleotidesBPHYT_RS26300 and BPHYT_RS26305 overlap by 4 nucleotides BPHYT_RS26290: BPHYT_RS26290 - photosystem reaction center subunit H, at 1,470,062 to 1,470,469 _RS26290 BPHYT_RS26295: BPHYT_RS26295 - chemotaxis protein CheY, at 1,470,677 to 1,471,135 _RS26295 BPHYT_RS26300: BPHYT_RS26300 - ATP-dependent DNA ligase, at 1,471,356 to 1,473,032 _RS26300 BPHYT_RS26305: BPHYT_RS26305 - exonuclease, at 1,473,029 to 1,474,177 _RS26305 Position (kb) 1471 1472 1473 1474Strain fitness (log2 ratio) -1 0 1 2at 1471.290 kb on - strandat 1471.416 kb on - strandat 1471.869 kb on + strand, within BPHYT_RS26300at 1472.654 kb on - strand, within BPHYT_RS26300at 1472.953 kb on + strandat 1473.011 kb on - strandat 1473.674 kb on - strand, within BPHYT_RS26305at 1474.011 kb on + strand, within BPHYT_RS26305

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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1,471,290 - +0.3
1,471,416 - +0.6
1,471,869 + BPHYT_RS26300 0.31 -0.2
1,472,654 - BPHYT_RS26300 0.77 +1.9
1,472,953 + -0.4
1,473,011 - +0.5
1,473,674 - BPHYT_RS26305 0.56 -0.9
1,474,011 + BPHYT_RS26305 0.85 -0.1

Or see this region's nucleotide sequence