Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26220 and BPHYT_RS26225 overlap by 10 nucleotides BPHYT_RS26225 and BPHYT_RS26230 are separated by 77 nucleotides BPHYT_RS26230 and BPHYT_RS26235 are separated by 172 nucleotides BPHYT_RS26235 and BPHYT_RS26240 are separated by 145 nucleotides
BPHYT_RS26220: BPHYT_RS26220 - 6-phosphogluconate dehydrogenase, at 1,454,569 to 1,455,606
_RS26220
BPHYT_RS26225: BPHYT_RS26225 - hypothetical protein, at 1,455,597 to 1,455,917
_RS26225
BPHYT_RS26230: BPHYT_RS26230 - MFS transporter, at 1,455,995 to 1,457,329
_RS26230
BPHYT_RS26235: BPHYT_RS26235 - hypothetical protein, at 1,457,502 to 1,458,038
_RS26235
BPHYT_RS26240: BPHYT_RS26240 - hypothetical protein, at 1,458,184 to 1,458,480
_RS26240
Position (kb)
1455
1456
1457
1458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1455.002 kb on + strand, within BPHYT_RS26220 at 1455.003 kb on - strand, within BPHYT_RS26220 at 1455.219 kb on - strand, within BPHYT_RS26220 at 1455.266 kb on + strand, within BPHYT_RS26220 at 1455.267 kb on - strand, within BPHYT_RS26220 at 1455.267 kb on - strand, within BPHYT_RS26220 at 1455.320 kb on + strand, within BPHYT_RS26220 at 1455.528 kb on + strand at 1455.668 kb on + strand, within BPHYT_RS26225 at 1455.945 kb on + strand at 1456.276 kb on - strand, within BPHYT_RS26230 at 1456.276 kb on - strand, within BPHYT_RS26230 at 1456.276 kb on - strand, within BPHYT_RS26230 at 1456.534 kb on + strand, within BPHYT_RS26230 at 1457.546 kb on - strand at 1457.546 kb on - strand at 1457.552 kb on - strand at 1457.552 kb on - strand at 1457.560 kb on + strand, within BPHYT_RS26235 at 1458.151 kb on + strand at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 1,455,002 + BPHYT_RS26220 0.42 +0.5 1,455,003 - BPHYT_RS26220 0.42 -2.8 1,455,219 - BPHYT_RS26220 0.63 -0.1 1,455,266 + BPHYT_RS26220 0.67 +0.2 1,455,267 - BPHYT_RS26220 0.67 +0.2 1,455,267 - BPHYT_RS26220 0.67 -1.1 1,455,320 + BPHYT_RS26220 0.72 -1.8 1,455,528 + -0.0 1,455,668 + BPHYT_RS26225 0.22 +0.2 1,455,945 + +0.3 1,456,276 - BPHYT_RS26230 0.21 +0.1 1,456,276 - BPHYT_RS26230 0.21 -0.1 1,456,276 - BPHYT_RS26230 0.21 -0.7 1,456,534 + BPHYT_RS26230 0.40 -0.8 1,457,546 - +3.9 1,457,546 - +0.3 1,457,552 - -0.3 1,457,552 - -0.4 1,457,560 + BPHYT_RS26235 0.11 +2.1 1,458,151 + -0.3 1,458,240 - BPHYT_RS26240 0.19 -0.4 1,458,240 - BPHYT_RS26240 0.19 -1.0 1,458,240 - BPHYT_RS26240 0.19 +0.6 1,458,240 - BPHYT_RS26240 0.19 +0.2
Or see this region's nucleotide sequence