Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26015 and BPHYT_RS26020 are separated by 35 nucleotides BPHYT_RS26020 and BPHYT_RS26025 are separated by 35 nucleotides BPHYT_RS26025 and BPHYT_RS26030 overlap by 10 nucleotides BPHYT_RS26030 and BPHYT_RS26035 overlap by 4 nucleotides
BPHYT_RS26015: BPHYT_RS26015 - rod shape-determining protein MreB, at 1,412,856 to 1,413,899
_RS26015
BPHYT_RS26020: BPHYT_RS26020 - GntR family transcriptional regulator, at 1,413,935 to 1,414,693
_RS26020
BPHYT_RS26025: BPHYT_RS26025 - hypothetical protein, at 1,414,729 to 1,415,124
_RS26025
BPHYT_RS26030: BPHYT_RS26030 - cysteine hydrolase, at 1,415,115 to 1,415,810
_RS26030
BPHYT_RS26035: BPHYT_RS26035 - ABC transporter ATP-binding protein, at 1,415,807 to 1,417,417
_RS26035
Position (kb) 1414
1415
1416 Strain fitness (log2 ratio) -1
0
1
2
3 at 1413.891 kb on + strand at 1414.372 kb on + strand, within BPHYT_RS26020 at 1415.070 kb on - strand, within BPHYT_RS26025 at 1415.236 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 1,413,891 + -1.4 1,414,372 + BPHYT_RS26020 0.58 -0.3 1,415,070 - BPHYT_RS26025 0.86 +1.8 1,415,236 + BPHYT_RS26030 0.17 +3.3 1,415,588 + BPHYT_RS26030 0.68 +0.8 1,415,588 + BPHYT_RS26030 0.68 +0.3
Or see this region's nucleotide sequence