Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21575 and BPHYT_RS21580 overlap by 11 nucleotides BPHYT_RS21580 and BPHYT_RS21585 are separated by 8 nucleotides BPHYT_RS21585 and BPHYT_RS21590 are separated by 40 nucleotides BPHYT_RS21590 and BPHYT_RS21595 are separated by 161 nucleotides BPHYT_RS21595 and BPHYT_RS21600 are separated by 183 nucleotides
BPHYT_RS21575: BPHYT_RS21575 - hypothetical protein, at 399,589 to 399,789
_RS21575
BPHYT_RS21580: BPHYT_RS21580 - hypothetical protein, at 399,779 to 399,967
_RS21580
BPHYT_RS21585: BPHYT_RS21585 - short-chain dehydrogenase, at 399,976 to 400,737
_RS21585
BPHYT_RS21590: BPHYT_RS21590 - NmrA family transcriptional regulator, at 400,778 to 401,656
_RS21590
BPHYT_RS21595: BPHYT_RS21595 - TetR family transcriptional regulator, at 401,818 to 402,390
_RS21595
BPHYT_RS21600: BPHYT_RS21600 - 3-oxoacyl-ACP reductase, at 402,574 to 403,284
_RS21600
Position (kb) 400
401
402 Strain fitness (log2 ratio) -1
0
1
2 at 399.827 kb on + strand, within BPHYT_RS21580 at 400.167 kb on + strand, within BPHYT_RS21585 at 400.314 kb on - strand, within BPHYT_RS21585 at 400.329 kb on + strand, within BPHYT_RS21585 at 400.421 kb on - strand, within BPHYT_RS21585 at 400.710 kb on + strand at 401.077 kb on + strand, within BPHYT_RS21590 at 401.784 kb on - strand at 401.791 kb on + strand at 402.110 kb on - strand, within BPHYT_RS21595 at 402.173 kb on - strand, within BPHYT_RS21595 at 402.461 kb on + strand at 402.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 399,827 + BPHYT_RS21580 0.25 +0.9 400,167 + BPHYT_RS21585 0.25 -0.8 400,314 - BPHYT_RS21585 0.44 +0.3 400,329 + BPHYT_RS21585 0.46 -0.4 400,421 - BPHYT_RS21585 0.58 +0.9 400,710 + +0.4 401,077 + BPHYT_RS21590 0.34 -1.1 401,784 - -0.1 401,791 + +0.7 402,110 - BPHYT_RS21595 0.51 +2.7 402,173 - BPHYT_RS21595 0.62 -0.3 402,461 + -1.0 402,522 + -0.4
Or see this region's nucleotide sequence