Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS18990 and BPHYT_RS18995 are separated by 43 nucleotides BPHYT_RS18995 and BPHYT_RS19000 are separated by 69 nucleotides BPHYT_RS19000 and BPHYT_RS19005 are separated by 86 nucleotides BPHYT_RS19005 and BPHYT_RS19010 are separated by 85 nucleotides
BPHYT_RS18990: BPHYT_RS18990 - chemotaxis protein, at 4,263,429 to 4,265,282
_RS18990
BPHYT_RS18995: BPHYT_RS18995 - purine-binding chemotaxis protein, at 4,265,326 to 4,265,862
_RS18995
BPHYT_RS19000: BPHYT_RS19000 - chemotaxis protein CheA, at 4,265,932 to 4,268,232
_RS19000
BPHYT_RS19005: BPHYT_RS19005 - chemotaxis protein CheY, at 4,268,319 to 4,268,696
_RS19005
BPHYT_RS19010: BPHYT_RS19010 - flagellar motor protein MotB, at 4,268,782 to 4,269,789
_RS19010
Position (kb) 4265
4266
4267
4268
4269 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4265.202 kb on + strand at 4265.244 kb on + strand at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.604 kb on + strand, within BPHYT_RS18995 at 4265.605 kb on - strand, within BPHYT_RS18995 at 4265.605 kb on - strand, within BPHYT_RS18995 at 4265.697 kb on - strand, within BPHYT_RS18995 at 4265.754 kb on + strand, within BPHYT_RS18995 at 4265.755 kb on - strand, within BPHYT_RS18995 at 4265.755 kb on - strand, within BPHYT_RS18995 at 4265.808 kb on + strand, within BPHYT_RS18995 at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.899 kb on + strand at 4265.900 kb on - strand at 4265.900 kb on - strand at 4265.900 kb on - strand at 4265.906 kb on + strand at 4266.224 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.269 kb on + strand, within BPHYT_RS19000 at 4266.270 kb on - strand, within BPHYT_RS19000 at 4266.329 kb on - strand, within BPHYT_RS19000 at 4266.329 kb on - strand, within BPHYT_RS19000 at 4266.329 kb on - strand, within BPHYT_RS19000 at 4266.905 kb on + strand, within BPHYT_RS19000 at 4266.906 kb on - strand, within BPHYT_RS19000 at 4266.906 kb on - strand, within BPHYT_RS19000 at 4267.281 kb on + strand, within BPHYT_RS19000 at 4267.751 kb on - strand, within BPHYT_RS19000 at 4267.998 kb on + strand, within BPHYT_RS19000 at 4268.207 kb on + strand at 4268.208 kb on - strand at 4268.300 kb on - strand at 4268.300 kb on - strand at 4268.309 kb on + strand at 4268.309 kb on + strand at 4268.310 kb on - strand at 4268.320 kb on + strand at 4268.450 kb on + strand, within BPHYT_RS19005 at 4268.451 kb on - strand, within BPHYT_RS19005 at 4268.533 kb on + strand, within BPHYT_RS19005 at 4268.533 kb on + strand, within BPHYT_RS19005 at 4268.533 kb on + strand, within BPHYT_RS19005 at 4268.534 kb on - strand, within BPHYT_RS19005 at 4268.534 kb on - strand, within BPHYT_RS19005 at 4268.534 kb on - strand, within BPHYT_RS19005 at 4268.721 kb on - strand at 4268.721 kb on - strand at 4269.107 kb on + strand, within BPHYT_RS19010 at 4269.108 kb on - strand, within BPHYT_RS19010 at 4269.128 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010 at 4269.218 kb on + strand, within BPHYT_RS19010
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 4,265,202 + +0.2 4,265,244 + -0.4 4,265,604 + BPHYT_RS18995 0.52 +0.1 4,265,604 + BPHYT_RS18995 0.52 +0.6 4,265,604 + BPHYT_RS18995 0.52 -2.0 4,265,605 - BPHYT_RS18995 0.52 +0.9 4,265,605 - BPHYT_RS18995 0.52 -0.3 4,265,697 - BPHYT_RS18995 0.69 -1.1 4,265,754 + BPHYT_RS18995 0.80 -1.0 4,265,755 - BPHYT_RS18995 0.80 +0.1 4,265,755 - BPHYT_RS18995 0.80 -0.3 4,265,808 + BPHYT_RS18995 0.90 +0.7 4,265,899 + -0.8 4,265,899 + +0.2 4,265,899 + -0.8 4,265,899 + +0.4 4,265,900 - -2.1 4,265,900 - +0.6 4,265,900 - -0.6 4,265,906 + +1.8 4,266,224 + BPHYT_RS19000 0.13 +0.8 4,266,269 + BPHYT_RS19000 0.15 -2.1 4,266,269 + BPHYT_RS19000 0.15 -0.6 4,266,269 + +1.3 4,266,269 + BPHYT_RS19000 0.15 +0.0 4,266,269 + BPHYT_RS19000 0.15 -0.5 4,266,269 + BPHYT_RS19000 0.15 +0.5 4,266,270 - BPHYT_RS19000 0.15 -0.5 4,266,329 - BPHYT_RS19000 0.17 +0.7 4,266,329 - BPHYT_RS19000 0.17 +0.5 4,266,329 - BPHYT_RS19000 0.17 -0.4 4,266,905 + BPHYT_RS19000 0.42 +0.4 4,266,906 - BPHYT_RS19000 0.42 -0.2 4,266,906 - BPHYT_RS19000 0.42 -0.3 4,267,281 + BPHYT_RS19000 0.59 +0.9 4,267,751 - BPHYT_RS19000 0.79 -0.0 4,267,998 + BPHYT_RS19000 0.90 -1.5 4,268,207 + +0.8 4,268,208 - +0.4 4,268,300 - -1.8 4,268,300 - +1.1 4,268,309 + -0.0 4,268,309 + -1.3 4,268,310 - +0.3 4,268,320 + +1.4 4,268,450 + BPHYT_RS19005 0.35 -0.4 4,268,451 - BPHYT_RS19005 0.35 +1.0 4,268,533 + BPHYT_RS19005 0.57 +0.2 4,268,533 + BPHYT_RS19005 0.57 +0.1 4,268,533 + BPHYT_RS19005 0.57 +0.1 4,268,534 - BPHYT_RS19005 0.57 +0.1 4,268,534 - BPHYT_RS19005 0.57 -0.0 4,268,534 - BPHYT_RS19005 0.57 +1.9 4,268,721 - -0.3 4,268,721 - +0.7 4,269,107 + BPHYT_RS19010 0.32 -1.2 4,269,108 - BPHYT_RS19010 0.32 +0.3 4,269,128 + BPHYT_RS19010 0.34 -0.0 4,269,218 + BPHYT_RS19010 0.43 +0.9 4,269,218 + BPHYT_RS19010 0.43 +0.3 4,269,218 + BPHYT_RS19010 0.43 +0.1 4,269,218 + BPHYT_RS19010 0.43 -0.3
Or see this region's nucleotide sequence