Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS15320

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS15310 and BPHYT_RS15315 are separated by 60 nucleotidesBPHYT_RS15315 and BPHYT_RS15320 are separated by 18 nucleotidesBPHYT_RS15320 and BPHYT_RS15325 are separated by 210 nucleotidesBPHYT_RS15325 and BPHYT_RS15330 overlap by 8 nucleotides BPHYT_RS15310: BPHYT_RS15310 - DNA-directed RNA polymerase subunit omega, at 3,479,069 to 3,479,272 _RS15310 BPHYT_RS15315: BPHYT_RS15315 - guanylate kinase, at 3,479,333 to 3,480,013 _RS15315 BPHYT_RS15320: BPHYT_RS15320 - hypothetical protein, at 3,480,032 to 3,480,955 _RS15320 BPHYT_RS15325: BPHYT_RS15325 - ribonuclease PH, at 3,481,166 to 3,481,906 _RS15325 BPHYT_RS15330: BPHYT_RS15330 - nucleoside-triphosphate diphosphatase, at 3,481,899 to 3,482,546 _RS15330 Position (kb) 3480 3481Strain fitness (log2 ratio) -1 0 1 2at 3479.309 kb on + strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3479.310 kb on - strandat 3480.034 kb on - strandat 3480.263 kb on - strand, within BPHYT_RS15320at 3480.274 kb on - strand, within BPHYT_RS15320at 3480.274 kb on - strand, within BPHYT_RS15320at 3480.274 kb on - strand, within BPHYT_RS15320at 3480.949 kb on - strandat 3480.949 kb on - strandat 3480.959 kb on - strandat 3481.048 kb on - strandat 3481.064 kb on + strandat 3481.065 kb on - strandat 3481.367 kb on + strand, within BPHYT_RS15325at 3481.652 kb on + strand, within BPHYT_RS15325at 3481.680 kb on - strand, within BPHYT_RS15325at 3481.680 kb on - strand, within BPHYT_RS15325at 3481.858 kb on + strandat 3481.858 kb on + strandat 3481.859 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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3,479,309 + +2.3
3,479,310 - -1.4
3,479,310 - +1.5
3,479,310 - -0.6
3,479,310 - -1.1
3,479,310 - +0.5
3,479,310 - +0.0
3,479,310 - -0.1
3,480,034 - +0.7
3,480,263 - BPHYT_RS15320 0.25 +0.5
3,480,274 - BPHYT_RS15320 0.26 +1.5
3,480,274 - BPHYT_RS15320 0.26 +1.3
3,480,274 - BPHYT_RS15320 0.26 -0.5
3,480,949 - +0.2
3,480,949 - +0.2
3,480,959 - +0.3
3,481,048 - -0.4
3,481,064 + -0.5
3,481,065 - +1.3
3,481,367 + BPHYT_RS15325 0.27 -1.0
3,481,652 + BPHYT_RS15325 0.66 +0.7
3,481,680 - BPHYT_RS15325 0.69 +0.6
3,481,680 - BPHYT_RS15325 0.69 +1.3
3,481,858 + -0.8
3,481,858 + +0.6
3,481,859 - +0.9

Or see this region's nucleotide sequence