Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15310 and BPHYT_RS15315 are separated by 60 nucleotides BPHYT_RS15315 and BPHYT_RS15320 are separated by 18 nucleotides BPHYT_RS15320 and BPHYT_RS15325 are separated by 210 nucleotides BPHYT_RS15325 and BPHYT_RS15330 overlap by 8 nucleotides
BPHYT_RS15310: BPHYT_RS15310 - DNA-directed RNA polymerase subunit omega, at 3,479,069 to 3,479,272
_RS15310
BPHYT_RS15315: BPHYT_RS15315 - guanylate kinase, at 3,479,333 to 3,480,013
_RS15315
BPHYT_RS15320: BPHYT_RS15320 - hypothetical protein, at 3,480,032 to 3,480,955
_RS15320
BPHYT_RS15325: BPHYT_RS15325 - ribonuclease PH, at 3,481,166 to 3,481,906
_RS15325
BPHYT_RS15330: BPHYT_RS15330 - nucleoside-triphosphate diphosphatase, at 3,481,899 to 3,482,546
_RS15330
Position (kb)
3480
3481 Strain fitness (log2 ratio)
-1
0
1
2 at 3479.309 kb on + strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3479.310 kb on - strand at 3480.034 kb on - strand at 3480.263 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.274 kb on - strand, within BPHYT_RS15320 at 3480.949 kb on - strand at 3480.949 kb on - strand at 3480.959 kb on - strand at 3481.048 kb on - strand at 3481.064 kb on + strand at 3481.065 kb on - strand at 3481.367 kb on + strand, within BPHYT_RS15325 at 3481.652 kb on + strand, within BPHYT_RS15325 at 3481.680 kb on - strand, within BPHYT_RS15325 at 3481.680 kb on - strand, within BPHYT_RS15325 at 3481.858 kb on + strand at 3481.858 kb on + strand at 3481.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 3,479,309 + +2.3 3,479,310 - -1.4 3,479,310 - +1.5 3,479,310 - -0.6 3,479,310 - -1.1 3,479,310 - +0.5 3,479,310 - +0.0 3,479,310 - -0.1 3,480,034 - +0.7 3,480,263 - BPHYT_RS15320 0.25 +0.5 3,480,274 - BPHYT_RS15320 0.26 +1.5 3,480,274 - BPHYT_RS15320 0.26 +1.3 3,480,274 - BPHYT_RS15320 0.26 -0.5 3,480,949 - +0.2 3,480,949 - +0.2 3,480,959 - +0.3 3,481,048 - -0.4 3,481,064 + -0.5 3,481,065 - +1.3 3,481,367 + BPHYT_RS15325 0.27 -1.0 3,481,652 + BPHYT_RS15325 0.66 +0.7 3,481,680 - BPHYT_RS15325 0.69 +0.6 3,481,680 - BPHYT_RS15325 0.69 +1.3 3,481,858 + -0.8 3,481,858 + +0.6 3,481,859 - +0.9
Or see this region's nucleotide sequence