Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13475

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13465 and BPHYT_RS13470 are separated by 94 nucleotidesBPHYT_RS13470 and BPHYT_RS13475 are separated by 155 nucleotidesBPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotidesBPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotides BPHYT_RS13465: BPHYT_RS13465 - hypothetical protein, at 3,074,444 to 3,074,824 _RS13465 BPHYT_RS13470: BPHYT_RS13470 - arylsulfate sulfotransferase, at 3,074,919 to 3,075,269 _RS13470 BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011 _RS13475 BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853 _RS13480 BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364 _RS13485 Position (kb) 3075 3076 3077 3078Strain fitness (log2 ratio) -2 -1 0 1at 3074.563 kb on - strand, within BPHYT_RS13465at 3074.631 kb on + strand, within BPHYT_RS13465at 3074.632 kb on - strand, within BPHYT_RS13465at 3074.765 kb on - strand, within BPHYT_RS13465at 3074.839 kb on + strandat 3075.022 kb on + strand, within BPHYT_RS13470at 3075.257 kb on - strandat 3075.259 kb on - strandat 3075.350 kb on + strandat 3075.688 kb on - strand, within BPHYT_RS13475at 3075.861 kb on + strand, within BPHYT_RS13475at 3075.867 kb on + strand, within BPHYT_RS13475at 3075.868 kb on - strand, within BPHYT_RS13475at 3076.093 kb on + strand, within BPHYT_RS13475at 3076.249 kb on - strand, within BPHYT_RS13475at 3076.508 kb on + strand, within BPHYT_RS13475at 3077.094 kb on + strandat 3077.136 kb on - strandat 3077.183 kb on + strandat 3077.929 kb on - strandat 3077.937 kb on + strandat 3077.964 kb on + strandat 3077.964 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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3,074,563 - BPHYT_RS13465 0.31 +0.6
3,074,631 + BPHYT_RS13465 0.49 -1.7
3,074,632 - BPHYT_RS13465 0.49 +0.4
3,074,765 - BPHYT_RS13465 0.84 -0.0
3,074,839 + +0.9
3,075,022 + BPHYT_RS13470 0.29 +0.6
3,075,257 - -0.3
3,075,259 - -1.7
3,075,350 + -0.2
3,075,688 - BPHYT_RS13475 0.17 +0.2
3,075,861 + BPHYT_RS13475 0.27 -0.3
3,075,867 + BPHYT_RS13475 0.28 +0.9
3,075,868 - BPHYT_RS13475 0.28 -0.8
3,076,093 + BPHYT_RS13475 0.42 -0.2
3,076,249 - BPHYT_RS13475 0.52 -0.1
3,076,508 + BPHYT_RS13475 0.68 -0.6
3,077,094 + -0.4
3,077,136 - +0.7
3,077,183 + -0.7
3,077,929 - -0.9
3,077,937 + +0.7
3,077,964 + -2.3
3,077,964 + +0.2

Or see this region's nucleotide sequence