Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13190 and BPHYT_RS13195 are separated by 364 nucleotides BPHYT_RS13195 and BPHYT_RS13200 are separated by 117 nucleotides BPHYT_RS13200 and BPHYT_RS13205 are separated by 40 nucleotides BPHYT_RS13205 and BPHYT_RS13210 are separated by 30 nucleotides
BPHYT_RS13190: BPHYT_RS13190 - membrane protein, at 3,010,573 to 3,011,502
_RS13190
BPHYT_RS13195: BPHYT_RS13195 - hypothetical protein, at 3,011,867 to 3,012,112
_RS13195
BPHYT_RS13200: BPHYT_RS13200 - molybdopterin binding oxidoreductase, at 3,012,230 to 3,013,021
_RS13200
BPHYT_RS13205: BPHYT_RS13205 - cytochrome B561, at 3,013,062 to 3,013,703
_RS13205
BPHYT_RS13210: BPHYT_RS13210 - hypothetical protein, at 3,013,734 to 3,014,579
_RS13210
Position (kb)
3012
3013
3014 Strain fitness (log2 ratio)
-2
-1
0
1 at 3011.308 kb on + strand, within BPHYT_RS13190 at 3011.384 kb on + strand, within BPHYT_RS13190 at 3011.731 kb on + strand at 3011.732 kb on - strand at 3012.153 kb on - strand at 3012.454 kb on - strand, within BPHYT_RS13200 at 3012.483 kb on + strand, within BPHYT_RS13200 at 3013.304 kb on + strand, within BPHYT_RS13205
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 3,011,308 + BPHYT_RS13190 0.79 +0.6 3,011,384 + BPHYT_RS13190 0.87 +0.1 3,011,731 + -0.6 3,011,732 - -2.2 3,012,153 - -0.2 3,012,454 - BPHYT_RS13200 0.28 +1.0 3,012,483 + BPHYT_RS13200 0.32 +0.3 3,013,304 + BPHYT_RS13205 0.38 -0.0
Or see this region's nucleotide sequence