Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11640

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11635 and BPHYT_RS11640 are separated by 11 nucleotidesBPHYT_RS11640 and BPHYT_RS11645 are separated by 285 nucleotidesBPHYT_RS11645 and BPHYT_RS11650 are separated by 151 nucleotides BPHYT_RS11635: BPHYT_RS11635 - RNA polymerase subunit sigma-24, at 2,641,686 to 2,642,567 _RS11635 BPHYT_RS11640: BPHYT_RS11640 - hypothetical protein, at 2,642,579 to 2,643,016 _RS11640 BPHYT_RS11645: BPHYT_RS11645 - hypothetical protein, at 2,643,302 to 2,643,826 _RS11645 BPHYT_RS11650: BPHYT_RS11650 - hypothetical protein, at 2,643,978 to 2,644,883 _RS11650 Position (kb) 2642 2643 2644Strain fitness (log2 ratio) -2 -1 0 1 2at 2641.579 kb on + strandat 2641.843 kb on - strand, within BPHYT_RS11635at 2642.553 kb on + strandat 2642.857 kb on - strand, within BPHYT_RS11640at 2642.922 kb on + strand, within BPHYT_RS11640at 2643.158 kb on + strandat 2643.161 kb on + strandat 2643.569 kb on - strand, within BPHYT_RS11645at 2643.998 kb on - strandat 2644.002 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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2,641,579 + -0.3
2,641,843 - BPHYT_RS11635 0.18 -0.5
2,642,553 + +1.5
2,642,857 - BPHYT_RS11640 0.63 -2.0
2,642,922 + BPHYT_RS11640 0.78 +1.2
2,643,158 + -1.2
2,643,161 + +0.4
2,643,569 - BPHYT_RS11645 0.51 +1.9
2,643,998 - +0.2
2,644,002 - -0.4

Or see this region's nucleotide sequence