Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11120 and BPHYT_RS11125 are separated by 239 nucleotides BPHYT_RS11125 and BPHYT_RS11130 are separated by 36 nucleotides BPHYT_RS11130 and BPHYT_RS11135 are separated by 325 nucleotides
BPHYT_RS11120: BPHYT_RS11120 - ferredoxin--NADP reductase, at 2,526,932 to 2,527,981
_RS11120
BPHYT_RS11125: BPHYT_RS11125 - alkylhydroperoxidase, at 2,528,221 to 2,528,658
_RS11125
BPHYT_RS11130: BPHYT_RS11130 - chemotaxis protein, at 2,528,695 to 2,530,269
_RS11130
BPHYT_RS11135: BPHYT_RS11135 - LysR family transcriptional regulator, at 2,530,595 to 2,531,455
_RS11135
Position (kb)
2528
2529
2530
2531 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2528.002 kb on + strand at 2528.003 kb on - strand at 2528.003 kb on - strand at 2528.243 kb on - strand at 2528.369 kb on - strand, within BPHYT_RS11125 at 2528.823 kb on + strand at 2528.920 kb on - strand, within BPHYT_RS11130 at 2529.985 kb on - strand, within BPHYT_RS11130 at 2530.311 kb on - strand at 2530.311 kb on - strand at 2530.354 kb on + strand at 2530.549 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,528,002 + +1.2 2,528,003 - -0.7 2,528,003 - +2.2 2,528,243 - -1.9 2,528,369 - BPHYT_RS11125 0.34 -0.7 2,528,823 + -0.6 2,528,920 - BPHYT_RS11130 0.14 -0.9 2,529,985 - BPHYT_RS11130 0.82 -1.4 2,530,311 - +0.4 2,530,311 - -0.6 2,530,354 + -1.3 2,530,549 + +0.2
Or see this region's nucleotide sequence