Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10710 and BPHYT_RS10715 are separated by 236 nucleotides BPHYT_RS10715 and BPHYT_RS10720 are separated by 67 nucleotides BPHYT_RS10720 and BPHYT_RS10725 are separated by 18 nucleotides BPHYT_RS10725 and BPHYT_RS10730 overlap by 4 nucleotides
BPHYT_RS10710: BPHYT_RS10710 - sensor histidine kinase, at 2,437,256 to 2,438,776
_RS10710
BPHYT_RS10715: BPHYT_RS10715 - acyl-CoA dehydrogenase, at 2,439,013 to 2,440,191
_RS10715
BPHYT_RS10720: BPHYT_RS10720 - hypothetical protein, at 2,440,259 to 2,440,636
_RS10720
BPHYT_RS10725: BPHYT_RS10725 - polyketide cyclase, at 2,440,655 to 2,441,170
_RS10725
BPHYT_RS10730: BPHYT_RS10730 - Glu-tRNA amidotransferase, at 2,441,167 to 2,442,291
_RS10730
Position (kb)
2439
2440
2441 Strain fitness (log2 ratio)
-1
0
1 at 2438.807 kb on + strand at 2438.927 kb on + strand at 2439.333 kb on + strand, within BPHYT_RS10715 at 2439.698 kb on - strand, within BPHYT_RS10715 at 2440.136 kb on - strand at 2440.136 kb on - strand at 2440.189 kb on + strand at 2440.217 kb on - strand at 2440.475 kb on + strand, within BPHYT_RS10720 at 2440.554 kb on - strand, within BPHYT_RS10720 at 2440.748 kb on - strand, within BPHYT_RS10725 at 2441.018 kb on + strand, within BPHYT_RS10725
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 2,438,807 + -1.4 2,438,927 + +0.6 2,439,333 + BPHYT_RS10715 0.27 -0.2 2,439,698 - BPHYT_RS10715 0.58 +0.3 2,440,136 - -0.3 2,440,136 - -0.7 2,440,189 + +1.0 2,440,217 - -0.5 2,440,475 + BPHYT_RS10720 0.57 +0.4 2,440,554 - BPHYT_RS10720 0.78 -1.0 2,440,748 - BPHYT_RS10725 0.18 +0.4 2,441,018 + BPHYT_RS10725 0.70 -0.1
Or see this region's nucleotide sequence