Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07715 and BPHYT_RS07720 are separated by 7 nucleotides BPHYT_RS07720 and BPHYT_RS07725 are separated by 67 nucleotides BPHYT_RS07725 and BPHYT_RS07730 are separated by 126 nucleotides
BPHYT_RS07715: BPHYT_RS07715 - N-succinylarginine dihydrolase, at 1,755,296 to 1,756,636
_RS07715
BPHYT_RS07720: BPHYT_RS07720 - Succinylglutamate desuccinylase (EC 3.5.1.96) (from data) , at 1,756,644 to 1,757,672
_RS07720
BPHYT_RS07725: BPHYT_RS07725 - glutathione S-transferase, at 1,757,740 to 1,758,360
_RS07725
BPHYT_RS07730: BPHYT_RS07730 - LysR family transcriptional regulator, at 1,758,487 to 1,759,395
_RS07730
Position (kb)
1756
1757
1758 Strain fitness (log2 ratio)
-1
0
1
2 at 1755.998 kb on + strand, within BPHYT_RS07715 at 1757.219 kb on + strand, within BPHYT_RS07720 at 1757.220 kb on - strand, within BPHYT_RS07720 at 1757.260 kb on - strand, within BPHYT_RS07720 at 1757.281 kb on + strand, within BPHYT_RS07720 at 1757.281 kb on + strand, within BPHYT_RS07720 at 1757.281 kb on + strand, within BPHYT_RS07720 at 1757.553 kb on + strand, within BPHYT_RS07720 at 1757.679 kb on - strand at 1757.742 kb on - strand at 1757.837 kb on + strand, within BPHYT_RS07725 at 1757.892 kb on - strand, within BPHYT_RS07725 at 1757.892 kb on - strand, within BPHYT_RS07725 at 1757.925 kb on - strand, within BPHYT_RS07725 at 1758.062 kb on + strand, within BPHYT_RS07725 at 1758.063 kb on - strand, within BPHYT_RS07725 at 1758.155 kb on - strand, within BPHYT_RS07725 at 1758.233 kb on + strand, within BPHYT_RS07725 at 1758.234 kb on - strand, within BPHYT_RS07725 at 1758.348 kb on - strand at 1758.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 1,755,998 + BPHYT_RS07715 0.52 +1.7 1,757,219 + BPHYT_RS07720 0.56 +0.2 1,757,220 - BPHYT_RS07720 0.56 +1.5 1,757,260 - BPHYT_RS07720 0.60 +1.2 1,757,281 + BPHYT_RS07720 0.62 +1.0 1,757,281 + BPHYT_RS07720 0.62 +0.8 1,757,281 + BPHYT_RS07720 0.62 -0.1 1,757,553 + BPHYT_RS07720 0.88 +1.2 1,757,679 - -0.1 1,757,742 - -0.2 1,757,837 + BPHYT_RS07725 0.16 +2.7 1,757,892 - BPHYT_RS07725 0.24 +0.9 1,757,892 - BPHYT_RS07725 0.24 -0.9 1,757,925 - BPHYT_RS07725 0.30 -0.2 1,758,062 + BPHYT_RS07725 0.52 -1.3 1,758,063 - BPHYT_RS07725 0.52 -0.5 1,758,155 - BPHYT_RS07725 0.67 +2.2 1,758,233 + BPHYT_RS07725 0.79 -0.2 1,758,234 - BPHYT_RS07725 0.80 -0.5 1,758,348 - -1.6 1,758,506 - +0.1
Or see this region's nucleotide sequence