Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07450

Experiment: 24hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07445 and BPHYT_RS07450 are separated by 282 nucleotidesBPHYT_RS07450 and BPHYT_RS07455 overlap by 4 nucleotidesBPHYT_RS07455 and BPHYT_RS07460 are separated by 23 nucleotides BPHYT_RS07445: BPHYT_RS07445 - carbonate dehydratase, at 1,692,254 to 1,692,826 _RS07445 BPHYT_RS07450: BPHYT_RS07450 - histidine kinase, at 1,693,109 to 1,694,524 _RS07450 BPHYT_RS07455: BPHYT_RS07455 - transcriptional regulator, at 1,694,521 to 1,695,204 _RS07455 BPHYT_RS07460: BPHYT_RS07460 - hypothetical protein, at 1,695,228 to 1,695,581 _RS07460 Position (kb) 1693 1694 1695Strain fitness (log2 ratio) -1 0 1 2at 1692.518 kb on + strand, within BPHYT_RS07445at 1692.875 kb on + strandat 1694.056 kb on - strand, within BPHYT_RS07450at 1694.281 kb on - strand, within BPHYT_RS07450at 1695.162 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1
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1,692,518 + BPHYT_RS07445 0.46 -0.5
1,692,875 + +2.0
1,694,056 - BPHYT_RS07450 0.67 +0.2
1,694,281 - BPHYT_RS07450 0.83 +1.1
1,695,162 - +0.3

Or see this region's nucleotide sequence