Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02855 and BPHYT_RS02860 are separated by 78 nucleotides BPHYT_RS02860 and BPHYT_RS02865 overlap by 1 nucleotides BPHYT_RS02865 and BPHYT_RS02870 are separated by 87 nucleotides BPHYT_RS02870 and BPHYT_RS02875 are separated by 73 nucleotides
BPHYT_RS02855: BPHYT_RS02855 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 636,794 to 637,675
_RS02855
BPHYT_RS02860: BPHYT_RS02860 - membrane protein, at 637,754 to 638,386
_RS02860
BPHYT_RS02865: BPHYT_RS02865 - hypothetical protein, at 638,386 to 640,227
_RS02865
BPHYT_RS02870: BPHYT_RS02870 - formamidopyrimidine-DNA glycosylase, at 640,315 to 641,145
_RS02870
BPHYT_RS02875: BPHYT_RS02875 - adenine glycosylase, at 641,219 to 642,280
_RS02875
Position (kb) 638
639
640
641 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 638.164 kb on - strand, within BPHYT_RS02860 at 638.516 kb on - strand at 638.730 kb on - strand, within BPHYT_RS02865 at 638.730 kb on - strand, within BPHYT_RS02865 at 638.823 kb on - strand, within BPHYT_RS02865 at 638.986 kb on - strand, within BPHYT_RS02865 at 639.134 kb on + strand, within BPHYT_RS02865 at 639.791 kb on + strand, within BPHYT_RS02865 at 640.257 kb on + strand at 640.257 kb on + strand at 640.270 kb on - strand at 640.275 kb on - strand at 640.275 kb on - strand at 640.310 kb on + strand at 640.311 kb on - strand at 640.363 kb on + strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.364 kb on - strand at 640.402 kb on - strand, within BPHYT_RS02870 at 640.489 kb on + strand, within BPHYT_RS02870 at 640.489 kb on + strand, within BPHYT_RS02870 at 640.490 kb on - strand at 640.760 kb on + strand, within BPHYT_RS02870 at 640.761 kb on - strand, within BPHYT_RS02870 at 640.842 kb on + strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.843 kb on - strand, within BPHYT_RS02870 at 640.846 kb on + strand, within BPHYT_RS02870 at 640.846 kb on + strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 640.847 kb on - strand, within BPHYT_RS02870 at 641.020 kb on + strand, within BPHYT_RS02870 at 641.035 kb on + strand, within BPHYT_RS02870 at 641.036 kb on - strand, within BPHYT_RS02870 at 641.036 kb on - strand, within BPHYT_RS02870 at 641.119 kb on + strand at 641.119 kb on + strand at 641.120 kb on - strand at 641.144 kb on - strand at 641.144 kb on - strand at 641.166 kb on + strand at 641.166 kb on + strand at 641.167 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 638,164 - BPHYT_RS02860 0.65 -0.7 638,516 - +0.3 638,730 - BPHYT_RS02865 0.19 +3.3 638,730 - BPHYT_RS02865 0.19 +0.7 638,823 - BPHYT_RS02865 0.24 +2.3 638,986 - BPHYT_RS02865 0.33 +2.3 639,134 + BPHYT_RS02865 0.41 +2.3 639,791 + BPHYT_RS02865 0.76 +2.3 640,257 + -0.6 640,257 + -0.2 640,270 - -1.8 640,275 - +1.8 640,275 - -0.7 640,310 + -0.4 640,311 - -0.9 640,363 + -0.7 640,364 - -0.5 640,364 - -0.3 640,364 - -0.6 640,364 - -1.0 640,402 - BPHYT_RS02870 0.10 +0.5 640,489 + BPHYT_RS02870 0.21 +0.3 640,489 + BPHYT_RS02870 0.21 -0.7 640,490 - -0.1 640,760 + BPHYT_RS02870 0.54 +2.3 640,761 - BPHYT_RS02870 0.54 -0.8 640,842 + BPHYT_RS02870 0.63 -2.1 640,843 - BPHYT_RS02870 0.64 +1.5 640,843 - BPHYT_RS02870 0.64 +0.3 640,843 - BPHYT_RS02870 0.64 -1.7 640,843 - BPHYT_RS02870 0.64 -1.0 640,843 - BPHYT_RS02870 0.64 +0.4 640,846 + BPHYT_RS02870 0.64 -2.0 640,846 + BPHYT_RS02870 0.64 +1.1 640,847 - BPHYT_RS02870 0.64 -0.1 640,847 - BPHYT_RS02870 0.64 -0.7 640,847 - BPHYT_RS02870 0.64 +0.2 641,020 + BPHYT_RS02870 0.85 -0.1 641,035 + BPHYT_RS02870 0.87 -2.1 641,036 - BPHYT_RS02870 0.87 -0.5 641,036 - BPHYT_RS02870 0.87 +0.7 641,119 + +0.5 641,119 + -0.2 641,120 - +0.0 641,144 - +1.3 641,144 - +0.3 641,166 + -0.5 641,166 + +0.3 641,167 - -0.5
Or see this region's nucleotide sequence