Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01095 and BPHYT_RS01100 are separated by 9 nucleotides BPHYT_RS01100 and BPHYT_RS01105 overlap by 8 nucleotides
BPHYT_RS01095: BPHYT_RS01095 - hypothetical protein, at 254,041 to 254,490
_RS01095
BPHYT_RS01100: BPHYT_RS01100 - protein mobD, at 254,500 to 255,204
_RS01100
BPHYT_RS01105: BPHYT_RS01105 - hypothetical protein, at 255,197 to 255,916
_RS01105
Position (kb) 254
255 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 253.099 kb on + strand at 253.171 kb on - strand at 253.211 kb on + strand at 253.266 kb on + strand at 253.314 kb on + strand at 253.416 kb on + strand at 253.494 kb on - strand at 253.531 kb on + strand at 253.585 kb on + strand at 253.824 kb on - strand at 253.854 kb on + strand at 254.197 kb on + strand, within BPHYT_RS01095 at 254.536 kb on + strand at 254.536 kb on + strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.537 kb on - strand at 254.623 kb on - strand, within BPHYT_RS01100 at 254.672 kb on + strand at 254.673 kb on - strand, within BPHYT_RS01100 at 254.740 kb on + strand, within BPHYT_RS01100 at 254.962 kb on + strand, within BPHYT_RS01100 at 254.963 kb on - strand, within BPHYT_RS01100 at 254.965 kb on + strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.966 kb on - strand, within BPHYT_RS01100 at 254.993 kb on - strand, within BPHYT_RS01100 at 255.102 kb on - strand, within BPHYT_RS01100 at 255.166 kb on + strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.167 kb on - strand at 255.371 kb on + strand, within BPHYT_RS01105 at 255.374 kb on + strand, within BPHYT_RS01105 at 255.386 kb on + strand, within BPHYT_RS01105 at 255.387 kb on - strand, within BPHYT_RS01105
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 253,099 + -0.4 253,171 - +0.3 253,211 + -1.7 253,266 + +0.7 253,314 + -0.4 253,416 + +0.7 253,494 - -0.0 253,531 + +0.0 253,585 + +0.2 253,824 - -0.5 253,854 + +1.2 254,197 + BPHYT_RS01095 0.35 -1.3 254,536 + +0.7 254,536 + -0.5 254,537 - +0.3 254,537 - +0.3 254,537 - +0.6 254,623 - BPHYT_RS01100 0.17 -0.1 254,672 + +1.3 254,673 - BPHYT_RS01100 0.25 -0.0 254,740 + BPHYT_RS01100 0.34 -0.6 254,962 + BPHYT_RS01100 0.66 -0.3 254,963 - BPHYT_RS01100 0.66 -0.4 254,965 + BPHYT_RS01100 0.66 -0.5 254,966 - BPHYT_RS01100 0.66 +0.5 254,966 - BPHYT_RS01100 0.66 -0.7 254,993 - BPHYT_RS01100 0.70 -0.2 255,102 - BPHYT_RS01100 0.85 +1.0 255,166 + -3.4 255,167 - -0.3 255,167 - +0.4 255,167 - -0.2 255,371 + BPHYT_RS01105 0.24 -0.7 255,374 + BPHYT_RS01105 0.25 -0.6 255,386 + BPHYT_RS01105 0.26 -0.5 255,387 - BPHYT_RS01105 0.26 +0.6
Or see this region's nucleotide sequence