Experiment: 24hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00090 and BPHYT_RS00095 are separated by 687 nucleotides BPHYT_RS00095 and BPHYT_RS00100 are separated by 280 nucleotides
BPHYT_RS00090: BPHYT_RS00090 - reverse transcriptase, at 26,795 to 28,150
_RS00090
BPHYT_RS00095: BPHYT_RS00095 - AraC family transcriptional regulator, at 28,838 to 29,704
_RS00095
BPHYT_RS00100: BPHYT_RS00100 - ethanolamine permease, at 29,985 to 31,388
_RS00100
Position (kb) 28
29
30 Strain fitness (log2 ratio) -1
0
1
2 at 28.005 kb on - strand, within BPHYT_RS00090 at 28.119 kb on + strand at 28.142 kb on + strand at 28.247 kb on - strand at 28.286 kb on + strand at 28.286 kb on + strand at 28.287 kb on - strand at 28.337 kb on + strand at 28.374 kb on + strand at 28.394 kb on + strand at 28.394 kb on + strand at 28.395 kb on - strand at 28.395 kb on - strand at 28.457 kb on + strand at 28.457 kb on + strand at 28.457 kb on + strand at 28.458 kb on - strand at 28.458 kb on - strand at 28.458 kb on - strand at 28.458 kb on - strand at 28.540 kb on + strand at 28.541 kb on - strand at 28.577 kb on - strand at 28.678 kb on + strand at 28.681 kb on - strand at 28.726 kb on - strand at 28.726 kb on - strand at 28.726 kb on - strand at 28.728 kb on + strand at 28.729 kb on - strand at 28.731 kb on + strand at 28.731 kb on + strand at 28.731 kb on + strand at 28.731 kb on + strand at 28.732 kb on - strand at 28.732 kb on - strand at 28.736 kb on - strand at 28.737 kb on + strand at 28.737 kb on + strand at 28.738 kb on - strand at 28.742 kb on - strand at 28.742 kb on - strand at 28.743 kb on + strand at 28.743 kb on + strand at 28.744 kb on - strand at 28.834 kb on - strand at 28.834 kb on - strand at 28.905 kb on + strand at 28.997 kb on - strand, within BPHYT_RS00095 at 28.998 kb on + strand, within BPHYT_RS00095 at 28.998 kb on + strand, within BPHYT_RS00095 at 28.999 kb on - strand, within BPHYT_RS00095 at 29.245 kb on + strand, within BPHYT_RS00095 at 29.247 kb on + strand, within BPHYT_RS00095 at 29.247 kb on + strand, within BPHYT_RS00095 at 29.247 kb on + strand, within BPHYT_RS00095 at 29.248 kb on - strand, within BPHYT_RS00095 at 29.248 kb on - strand, within BPHYT_RS00095 at 29.248 kb on - strand, within BPHYT_RS00095 at 29.248 kb on - strand, within BPHYT_RS00095 at 29.280 kb on - strand, within BPHYT_RS00095 at 29.376 kb on + strand, within BPHYT_RS00095 at 29.527 kb on + strand, within BPHYT_RS00095 at 29.559 kb on + strand, within BPHYT_RS00095 at 29.559 kb on + strand, within BPHYT_RS00095 at 29.560 kb on - strand, within BPHYT_RS00095 at 29.578 kb on - strand, within BPHYT_RS00095 at 29.640 kb on + strand at 29.640 kb on + strand at 29.641 kb on - strand at 29.690 kb on - strand at 29.744 kb on + strand at 29.784 kb on + strand at 29.835 kb on + strand at 30.118 kb on - strand at 30.180 kb on + strand, within BPHYT_RS00100 at 30.181 kb on - strand, within BPHYT_RS00100 at 30.181 kb on - strand, within BPHYT_RS00100 at 30.182 kb on + strand, within BPHYT_RS00100 at 30.221 kb on + strand, within BPHYT_RS00100 at 30.409 kb on - strand, within BPHYT_RS00100
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 1 remove 28,005 - BPHYT_RS00090 0.89 -0.2 28,119 + +0.6 28,142 + +0.2 28,247 - -0.7 28,286 + -0.6 28,286 + +0.6 28,287 - -0.8 28,337 + -1.2 28,374 + -0.3 28,394 + -0.0 28,394 + +0.9 28,395 - +1.8 28,395 - +0.3 28,457 + -0.9 28,457 + -0.8 28,457 + +0.2 28,458 - +0.6 28,458 - +0.9 28,458 - -0.6 28,458 - +0.7 28,540 + +0.8 28,541 - -0.2 28,577 - +0.3 28,678 + -0.2 28,681 - -1.2 28,726 - -0.4 28,726 - -1.1 28,726 - -0.2 28,728 + -1.0 28,729 - -0.5 28,731 + +0.6 28,731 + -1.5 28,731 + +0.8 28,731 + +1.3 28,732 - -0.6 28,732 - -0.3 28,736 - -1.2 28,737 + -0.8 28,737 + -0.2 28,738 - -0.9 28,742 - -0.0 28,742 - +0.1 28,743 + -0.1 28,743 + +1.1 28,744 - -0.7 28,834 - -0.4 28,834 - +0.1 28,905 + +0.0 28,997 - BPHYT_RS00095 0.18 +0.6 28,998 + BPHYT_RS00095 0.18 -0.9 28,998 + BPHYT_RS00095 0.18 +0.7 28,999 - BPHYT_RS00095 0.19 -0.1 29,245 + BPHYT_RS00095 0.47 +0.9 29,247 + BPHYT_RS00095 0.47 +1.6 29,247 + BPHYT_RS00095 0.47 -0.6 29,247 + BPHYT_RS00095 0.47 +0.6 29,248 - BPHYT_RS00095 0.47 -0.6 29,248 - BPHYT_RS00095 0.47 +0.2 29,248 - BPHYT_RS00095 0.47 -0.7 29,248 - BPHYT_RS00095 0.47 +0.0 29,280 - BPHYT_RS00095 0.51 +1.0 29,376 + BPHYT_RS00095 0.62 -0.0 29,527 + BPHYT_RS00095 0.79 -0.1 29,559 + BPHYT_RS00095 0.83 +1.0 29,559 + BPHYT_RS00095 0.83 +1.8 29,560 - BPHYT_RS00095 0.83 -1.4 29,578 - BPHYT_RS00095 0.85 -1.3 29,640 + -0.8 29,640 + +0.5 29,641 - -0.1 29,690 - +0.0 29,744 + +0.3 29,784 + +0.5 29,835 + +0.6 30,118 - -0.8 30,180 + BPHYT_RS00100 0.14 +0.2 30,181 - BPHYT_RS00100 0.14 -0.0 30,181 - BPHYT_RS00100 0.14 -0.3 30,182 + BPHYT_RS00100 0.14 -0.4 30,221 + BPHYT_RS00100 0.17 -0.1 30,409 - BPHYT_RS00100 0.30 -0.4
Or see this region's nucleotide sequence