Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35795

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35785 and BPHYT_RS35790 are separated by 148 nucleotidesBPHYT_RS35790 and BPHYT_RS35795 are separated by 145 nucleotidesBPHYT_RS35795 and BPHYT_RS35800 are separated by 89 nucleotidesBPHYT_RS35800 and BPHYT_RS35805 are separated by 184 nucleotides BPHYT_RS35785: BPHYT_RS35785 - glucose-1-dehydrogenase, at 3,618,414 to 3,619,214 _RS35785 BPHYT_RS35790: BPHYT_RS35790 - hypothetical protein, at 3,619,363 to 3,619,518 _RS35790 BPHYT_RS35795: BPHYT_RS35795 - membrane protein, at 3,619,664 to 3,620,899 _RS35795 BPHYT_RS35800: BPHYT_RS35800 - TetR family transcriptional regulator, at 3,620,989 to 3,621,603 _RS35800 BPHYT_RS35805: BPHYT_RS35805 - DSBA oxidoreductase, at 3,621,788 to 3,623,359 _RS35805 Position (kb) 3619 3620 3621Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3618.725 kb on - strand, within BPHYT_RS35785at 3618.920 kb on + strand, within BPHYT_RS35785at 3619.237 kb on + strandat 3619.238 kb on - strandat 3619.262 kb on + strandat 3619.262 kb on + strandat 3619.262 kb on + strandat 3619.263 kb on - strandat 3619.340 kb on + strandat 3619.340 kb on + strandat 3619.341 kb on - strandat 3619.341 kb on - strandat 3619.350 kb on + strandat 3619.365 kb on - strandat 3619.366 kb on + strandat 3619.645 kb on - strandat 3619.780 kb on + strandat 3619.800 kb on + strand, within BPHYT_RS35795at 3620.170 kb on - strand, within BPHYT_RS35795at 3620.229 kb on + strand, within BPHYT_RS35795at 3620.388 kb on + strand, within BPHYT_RS35795at 3620.873 kb on - strandat 3621.443 kb on - strand, within BPHYT_RS35800at 3621.471 kb on - strand, within BPHYT_RS35800at 3621.531 kb on - strand, within BPHYT_RS35800at 3621.709 kb on + strandat 3621.709 kb on + strandat 3621.709 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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3,618,725 - BPHYT_RS35785 0.39 -2.4
3,618,920 + BPHYT_RS35785 0.63 +3.3
3,619,237 + +0.4
3,619,238 - +0.2
3,619,262 + -0.2
3,619,262 + -0.2
3,619,262 + +1.5
3,619,263 - +0.4
3,619,340 + +1.0
3,619,340 + +1.1
3,619,341 - -0.0
3,619,341 - -0.5
3,619,350 + +2.3
3,619,365 - +0.4
3,619,366 + -0.2
3,619,645 - +0.1
3,619,780 + -0.5
3,619,800 + BPHYT_RS35795 0.11 -0.4
3,620,170 - BPHYT_RS35795 0.41 -0.4
3,620,229 + BPHYT_RS35795 0.46 +0.3
3,620,388 + BPHYT_RS35795 0.59 +0.6
3,620,873 - -0.8
3,621,443 - BPHYT_RS35800 0.74 +0.5
3,621,471 - BPHYT_RS35800 0.78 -1.1
3,621,531 - BPHYT_RS35800 0.88 -1.2
3,621,709 + +0.4
3,621,709 + +0.1
3,621,709 + -0.6

Or see this region's nucleotide sequence