Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotides BPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotides BPHYT_RS28745 and BPHYT_RS28750 are separated by 65 nucleotides BPHYT_RS28750 and BPHYT_RS28755 are separated by 86 nucleotides
BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406
_RS28735
BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326
_RS28740
BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149
_RS28745
BPHYT_RS28750: BPHYT_RS28750 - IclR family transcriptional regulator, at 2,003,215 to 2,003,499
_RS28750
BPHYT_RS28755: BPHYT_RS28755 - 3-(2-hydroxyphenyl) propionic acid transporter, at 2,003,586 to 2,005,019
_RS28755
Position (kb)
2002
2003
2004 Strain fitness (log2 ratio)
-1
0
1 at 2001.406 kb on + strand at 2001.438 kb on - strand at 2001.480 kb on + strand at 2001.489 kb on - strand at 2001.609 kb on + strand, within BPHYT_RS28740 at 2001.651 kb on - strand, within BPHYT_RS28740 at 2001.810 kb on + strand, within BPHYT_RS28740 at 2002.428 kb on + strand, within BPHYT_RS28745 at 2002.778 kb on - strand, within BPHYT_RS28745 at 2002.782 kb on - strand, within BPHYT_RS28745 at 2002.843 kb on + strand, within BPHYT_RS28745 at 2003.426 kb on - strand, within BPHYT_RS28750 at 2003.549 kb on + strand at 2003.549 kb on + strand at 2003.549 kb on + strand at 2003.549 kb on + strand at 2003.550 kb on - strand at 2003.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 2,001,406 + +0.4 2,001,438 - +0.7 2,001,480 + -1.4 2,001,489 - +1.2 2,001,609 + BPHYT_RS28740 0.19 +0.1 2,001,651 - BPHYT_RS28740 0.24 +1.0 2,001,810 + BPHYT_RS28740 0.42 +1.7 2,002,428 + BPHYT_RS28745 0.11 +1.3 2,002,778 - BPHYT_RS28745 0.54 -0.3 2,002,782 - BPHYT_RS28745 0.55 +0.1 2,002,843 + BPHYT_RS28745 0.62 +0.6 2,003,426 - BPHYT_RS28750 0.74 -1.0 2,003,549 + -0.8 2,003,549 + -1.2 2,003,549 + -1.0 2,003,549 + -0.4 2,003,550 - +1.3 2,003,553 + -0.6
Or see this region's nucleotide sequence