Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS24060

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS24055 and BPHYT_RS24060 overlap by 4 nucleotidesBPHYT_RS24060 and BPHYT_RS24065 are separated by 25 nucleotides BPHYT_RS24055: BPHYT_RS24055 - oxidoreductase, at 960,641 to 961,630 _RS24055 BPHYT_RS24060: BPHYT_RS24060 - FAD-linked oxidase, at 961,627 to 963,039 _RS24060 BPHYT_RS24065: BPHYT_RS24065 - glucose-methanol-choline oxidoreductase, at 963,065 to 966,523 _RS24065 Position (kb) 961 962 963 964Strain fitness (log2 ratio) -3 -2 -1 0 1at 960.908 kb on + strand, within BPHYT_RS24055at 961.093 kb on - strand, within BPHYT_RS24055at 961.312 kb on + strand, within BPHYT_RS24055at 961.959 kb on + strand, within BPHYT_RS24060at 962.344 kb on + strand, within BPHYT_RS24060at 962.431 kb on + strand, within BPHYT_RS24060at 963.082 kb on - strandat 963.457 kb on - strand, within BPHYT_RS24065at 963.628 kb on + strand, within BPHYT_RS24065

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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960,908 + BPHYT_RS24055 0.27 -2.9
961,093 - BPHYT_RS24055 0.46 -0.3
961,312 + BPHYT_RS24055 0.68 -0.7
961,959 + BPHYT_RS24060 0.23 -1.9
962,344 + BPHYT_RS24060 0.51 -2.8
962,431 + BPHYT_RS24060 0.57 +0.1
963,082 - +0.2
963,457 - BPHYT_RS24065 0.11 +0.9
963,628 + BPHYT_RS24065 0.16 +0.4

Or see this region's nucleotide sequence