Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23310 and BPHYT_RS23315 are separated by 82 nucleotides BPHYT_RS23315 and BPHYT_RS23320 are separated by 123 nucleotides BPHYT_RS23320 and BPHYT_RS23325 are separated by 8 nucleotides BPHYT_RS23325 and BPHYT_RS23330 are separated by 400 nucleotides
BPHYT_RS23310: BPHYT_RS23310 - hypothetical protein, at 791,746 to 792,558
_RS23310
BPHYT_RS23315: BPHYT_RS23315 - hypothetical protein, at 792,641 to 793,042
_RS23315
BPHYT_RS23320: BPHYT_RS23320 - hypothetical protein, at 793,166 to 793,630
_RS23320
BPHYT_RS23325: BPHYT_RS23325 - ribonuclease, at 793,639 to 794,094
_RS23325
BPHYT_RS23330: BPHYT_RS23330 - hypothetical protein, at 794,495 to 794,680
_RS23330
Position (kb)
793
794 Strain fitness (log2 ratio)
-2
-1
0
1 at 792.181 kb on + strand, within BPHYT_RS23310 at 792.218 kb on - strand, within BPHYT_RS23310 at 792.590 kb on + strand at 792.613 kb on + strand at 792.897 kb on - strand, within BPHYT_RS23315 at 793.137 kb on + strand at 793.335 kb on + strand, within BPHYT_RS23320 at 793.435 kb on + strand, within BPHYT_RS23320 at 793.470 kb on - strand, within BPHYT_RS23320 at 793.477 kb on - strand, within BPHYT_RS23320 at 793.481 kb on + strand, within BPHYT_RS23320 at 793.617 kb on + strand at 793.641 kb on - strand at 793.683 kb on - strand at 793.721 kb on - strand, within BPHYT_RS23325 at 793.721 kb on - strand, within BPHYT_RS23325 at 793.769 kb on + strand, within BPHYT_RS23325 at 793.770 kb on - strand, within BPHYT_RS23325 at 794.138 kb on - strand at 794.138 kb on - strand at 794.144 kb on + strand at 794.387 kb on + strand at 794.405 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 792,181 + BPHYT_RS23310 0.54 -0.2 792,218 - BPHYT_RS23310 0.58 -2.0 792,590 + -0.8 792,613 + +0.7 792,897 - BPHYT_RS23315 0.64 -0.6 793,137 + +0.1 793,335 + BPHYT_RS23320 0.36 -0.6 793,435 + BPHYT_RS23320 0.58 +1.1 793,470 - BPHYT_RS23320 0.65 -0.2 793,477 - BPHYT_RS23320 0.67 -0.1 793,481 + BPHYT_RS23320 0.68 +0.6 793,617 + -0.6 793,641 - +0.5 793,683 - +1.1 793,721 - BPHYT_RS23325 0.18 +0.1 793,721 - BPHYT_RS23325 0.18 -0.9 793,769 + BPHYT_RS23325 0.29 -0.3 793,770 - BPHYT_RS23325 0.29 +0.3 794,138 - -0.4 794,138 - -0.3 794,144 + -0.6 794,387 + -0.4 794,405 + -0.5
Or see this region's nucleotide sequence