Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21210 and BPHYT_RS21215 are separated by 130 nucleotides BPHYT_RS21215 and BPHYT_RS21220 are separated by 403 nucleotides
BPHYT_RS21210: BPHYT_RS21210 - hypothetical protein, at 320,572 to 321,891
_RS21210
BPHYT_RS21215: BPHYT_RS21215 - Crp/Fnr family transcriptional regulator, at 322,022 to 322,678
_RS21215
BPHYT_RS21220: BPHYT_RS21220 - universal stress protein A, at 323,082 to 323,549
_RS21220
Position (kb) 322
323 Strain fitness (log2 ratio) -1
0
1
2
3 at 321.208 kb on - strand, within BPHYT_RS21210 at 321.407 kb on - strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.422 kb on + strand, within BPHYT_RS21210 at 321.423 kb on - strand, within BPHYT_RS21210 at 321.749 kb on + strand, within BPHYT_RS21210 at 322.023 kb on + strand at 322.024 kb on - strand at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.324 kb on + strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.325 kb on - strand, within BPHYT_RS21215 at 322.545 kb on + strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.546 kb on - strand, within BPHYT_RS21215 at 322.575 kb on + strand, within BPHYT_RS21215 at 322.813 kb on + strand at 322.813 kb on + strand at 322.813 kb on + strand at 322.845 kb on + strand at 322.846 kb on - strand at 322.991 kb on + strand at 323.416 kb on + strand, within BPHYT_RS21220 at 323.416 kb on + strand, within BPHYT_RS21220 at 323.417 kb on - strand, within BPHYT_RS21220 at 323.417 kb on - strand, within BPHYT_RS21220 at 323.417 kb on - strand, within BPHYT_RS21220 at 323.440 kb on + strand, within BPHYT_RS21220 at 323.440 kb on + strand, within BPHYT_RS21220 at 323.441 kb on - strand, within BPHYT_RS21220 at 323.442 kb on + strand, within BPHYT_RS21220 at 323.442 kb on + strand, within BPHYT_RS21220 at 323.545 kb on + strand at 323.545 kb on + strand at 323.545 kb on + strand at 323.545 kb on + strand at 323.545 kb on + strand at 323.546 kb on - strand at 323.546 kb on - strand at 323.627 kb on + strand at 323.627 kb on + strand at 323.628 kb on - strand at 323.628 kb on - strand at 323.670 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 321,208 - BPHYT_RS21210 0.48 -0.5 321,407 - BPHYT_RS21210 0.63 +1.7 321,422 + BPHYT_RS21210 0.64 +0.6 321,422 + BPHYT_RS21210 0.64 -1.1 321,423 - BPHYT_RS21210 0.64 -0.6 321,749 + BPHYT_RS21210 0.89 -0.5 322,023 + +0.2 322,024 - -0.5 322,324 + BPHYT_RS21215 0.46 -0.0 322,324 + BPHYT_RS21215 0.46 +1.7 322,324 + BPHYT_RS21215 0.46 +1.0 322,324 + BPHYT_RS21215 0.46 -0.7 322,325 - BPHYT_RS21215 0.46 +0.7 322,325 - BPHYT_RS21215 0.46 +0.0 322,545 + BPHYT_RS21215 0.80 -0.3 322,546 - BPHYT_RS21215 0.80 -0.3 322,546 - BPHYT_RS21215 0.80 +1.3 322,575 + BPHYT_RS21215 0.84 +0.5 322,813 + +1.7 322,813 + +1.5 322,813 + -1.3 322,845 + +0.2 322,846 - -0.0 322,991 + +3.3 323,416 + BPHYT_RS21220 0.71 +0.1 323,416 + BPHYT_RS21220 0.71 -0.6 323,417 - BPHYT_RS21220 0.72 -0.7 323,417 - BPHYT_RS21220 0.72 +2.3 323,417 - BPHYT_RS21220 0.72 -0.9 323,440 + BPHYT_RS21220 0.76 +0.0 323,440 + BPHYT_RS21220 0.76 +1.3 323,441 - BPHYT_RS21220 0.77 -0.3 323,442 + BPHYT_RS21220 0.77 +0.5 323,442 + BPHYT_RS21220 0.77 -0.5 323,545 + -1.1 323,545 + +0.1 323,545 + -0.7 323,545 + +0.6 323,545 + -0.8 323,546 - -0.1 323,546 - -0.8 323,627 + -0.4 323,627 + -0.9 323,628 - +1.5 323,628 - -1.2 323,670 + +0.3
Or see this region's nucleotide sequence