Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS03655

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS03650 and BPHYT_RS03655 are separated by 206 nucleotidesBPHYT_RS03655 and BPHYT_RS03660 are separated by 131 nucleotides BPHYT_RS03650: BPHYT_RS03650 - DNA mismatch repair protein, at 829,035 to 831,098 _RS03650 BPHYT_RS03655: BPHYT_RS03655 - membrane protein, at 831,305 to 831,976 _RS03655 BPHYT_RS03660: BPHYT_RS03660 - mechanosensitive ion channel protein MscS, at 832,108 to 832,920 _RS03660 Position (kb) 831 832Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 830.748 kb on - strand, within BPHYT_RS03650at 831.019 kb on - strandat 831.109 kb on + strandat 831.110 kb on - strandat 831.152 kb on + strandat 831.192 kb on + strandat 831.193 kb on - strandat 831.293 kb on - strandat 831.318 kb on + strandat 831.567 kb on - strand, within BPHYT_RS03655at 831.833 kb on + strand, within BPHYT_RS03655at 831.983 kb on - strandat 832.211 kb on + strand, within BPHYT_RS03660at 832.416 kb on - strand, within BPHYT_RS03660at 832.416 kb on - strand, within BPHYT_RS03660at 832.416 kb on - strand, within BPHYT_RS03660at 832.416 kb on - strand, within BPHYT_RS03660at 832.426 kb on - strand, within BPHYT_RS03660at 832.745 kb on + strand, within BPHYT_RS03660at 832.974 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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830,748 - BPHYT_RS03650 0.83 -0.0
831,019 - +1.9
831,109 + -0.3
831,110 - -0.2
831,152 + -0.4
831,192 + -0.5
831,193 - +0.5
831,293 - -2.5
831,318 + -3.0
831,567 - BPHYT_RS03655 0.39 -0.3
831,833 + BPHYT_RS03655 0.79 -2.6
831,983 - -0.0
832,211 + BPHYT_RS03660 0.13 +0.0
832,416 - BPHYT_RS03660 0.38 -0.2
832,416 - BPHYT_RS03660 0.38 -0.2
832,416 - BPHYT_RS03660 0.38 +2.0
832,416 - BPHYT_RS03660 0.38 -0.7
832,426 - BPHYT_RS03660 0.39 -0.6
832,745 + BPHYT_RS03660 0.78 -0.0
832,974 + +2.3

Or see this region's nucleotide sequence