Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34105

Experiment: 24hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34100 and BPHYT_RS34105 are separated by 129 nucleotidesBPHYT_RS34105 and BPHYT_RS34110 are separated by 6 nucleotides BPHYT_RS34100: BPHYT_RS34100 - spermidine/putrescine ABC transporter ATP-binding protein, at 3,248,375 to 3,249,472 _RS34100 BPHYT_RS34105: BPHYT_RS34105 - decarboxylase, at 3,249,602 to 3,251,149 _RS34105 BPHYT_RS34110: BPHYT_RS34110 - hypothetical protein, at 3,251,156 to 3,254,899 _RS34110 Position (kb) 3249 3250 3251 3252Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3248.794 kb on - strand, within BPHYT_RS34100at 3249.379 kb on - strandat 3249.379 kb on - strandat 3249.407 kb on + strandat 3249.423 kb on + strandat 3249.545 kb on + strandat 3249.545 kb on + strandat 3249.546 kb on - strandat 3249.547 kb on + strandat 3249.547 kb on + strandat 3249.563 kb on + strandat 3249.821 kb on + strand, within BPHYT_RS34105at 3249.822 kb on - strand, within BPHYT_RS34105at 3249.852 kb on - strand, within BPHYT_RS34105at 3250.515 kb on - strand, within BPHYT_RS34105at 3250.554 kb on - strand, within BPHYT_RS34105at 3250.677 kb on - strand, within BPHYT_RS34105at 3250.677 kb on - strand, within BPHYT_RS34105at 3250.827 kb on - strand, within BPHYT_RS34105at 3251.010 kb on - strandat 3251.233 kb on + strandat 3251.425 kb on - strandat 3251.425 kb on - strandat 3251.425 kb on - strandat 3251.425 kb on - strandat 3251.439 kb on + strandat 3251.439 kb on + strandat 3251.439 kb on + strandat 3251.439 kb on + strandat 3251.440 kb on - strandat 3251.440 kb on - strandat 3251.440 kb on - strandat 3251.448 kb on + strandat 3251.700 kb on + strand, within BPHYT_RS34110at 3252.004 kb on - strand, within BPHYT_RS34110at 3252.004 kb on - strand, within BPHYT_RS34110at 3252.051 kb on + strand, within BPHYT_RS34110at 3252.063 kb on + strand, within BPHYT_RS34110at 3252.063 kb on + strand, within BPHYT_RS34110

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10
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3,248,794 - BPHYT_RS34100 0.38 -0.8
3,249,379 - +0.5
3,249,379 - +0.4
3,249,407 + +0.2
3,249,423 + -0.4
3,249,545 + +1.7
3,249,545 + -0.5
3,249,546 - -1.0
3,249,547 + -0.8
3,249,547 + -0.1
3,249,563 + +0.1
3,249,821 + BPHYT_RS34105 0.14 +0.2
3,249,822 - BPHYT_RS34105 0.14 +1.1
3,249,852 - BPHYT_RS34105 0.16 +0.1
3,250,515 - BPHYT_RS34105 0.59 +2.0
3,250,554 - BPHYT_RS34105 0.61 +0.4
3,250,677 - BPHYT_RS34105 0.69 +1.7
3,250,677 - BPHYT_RS34105 0.69 +0.9
3,250,827 - BPHYT_RS34105 0.79 -0.2
3,251,010 - -0.1
3,251,233 + -0.3
3,251,425 - +0.4
3,251,425 - -0.4
3,251,425 - +0.2
3,251,425 - -0.6
3,251,439 + -1.1
3,251,439 + -0.8
3,251,439 + -0.3
3,251,439 + -0.5
3,251,440 - -1.3
3,251,440 - -0.1
3,251,440 - -0.9
3,251,448 + -1.1
3,251,700 + BPHYT_RS34110 0.15 +0.0
3,252,004 - BPHYT_RS34110 0.23 -1.7
3,252,004 - BPHYT_RS34110 0.23 -2.9
3,252,051 + BPHYT_RS34110 0.24 +0.0
3,252,063 + BPHYT_RS34110 0.24 +1.3
3,252,063 + BPHYT_RS34110 0.24 -0.7

Or see this region's nucleotide sequence