Experiment: 24hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34085 and BPHYT_RS34090 are separated by 15 nucleotides BPHYT_RS34090 and BPHYT_RS34095 are separated by 119 nucleotides
BPHYT_RS34085: BPHYT_RS34085 - polyamine ABC transporter permease, at 3,244,965 to 3,245,804
_RS34085
BPHYT_RS34090: BPHYT_RS34090 - polyamine ABC transporter substrate-binding protein, at 3,245,820 to 3,247,097
_RS34090
BPHYT_RS34095: BPHYT_RS34095 - spermidine/putrescine ABC transporter substrate-binding protein, at 3,247,217 to 3,248,266
_RS34095
Position (kb)
3245
3246
3247
3248 Strain fitness (log2 ratio)
-2
-1
0
1 at 3244.852 kb on + strand at 3244.902 kb on + strand at 3244.962 kb on + strand at 3244.963 kb on - strand at 3244.974 kb on - strand at 3245.019 kb on + strand at 3245.019 kb on + strand at 3245.079 kb on - strand, within BPHYT_RS34085 at 3245.117 kb on + strand, within BPHYT_RS34085 at 3245.375 kb on + strand, within BPHYT_RS34085 at 3245.771 kb on + strand at 3245.772 kb on - strand at 3245.866 kb on + strand at 3245.867 kb on - strand at 3245.956 kb on + strand, within BPHYT_RS34090 at 3246.005 kb on - strand, within BPHYT_RS34090 at 3246.005 kb on - strand, within BPHYT_RS34090 at 3246.073 kb on + strand, within BPHYT_RS34090 at 3246.074 kb on - strand, within BPHYT_RS34090 at 3246.074 kb on - strand, within BPHYT_RS34090 at 3246.158 kb on + strand, within BPHYT_RS34090 at 3246.159 kb on - strand, within BPHYT_RS34090 at 3246.160 kb on + strand, within BPHYT_RS34090 at 3246.160 kb on + strand, within BPHYT_RS34090 at 3246.161 kb on - strand, within BPHYT_RS34090 at 3246.161 kb on - strand, within BPHYT_RS34090 at 3246.487 kb on + strand, within BPHYT_RS34090 at 3246.488 kb on - strand, within BPHYT_RS34090 at 3246.885 kb on - strand, within BPHYT_RS34090 at 3247.168 kb on - strand at 3247.401 kb on + strand, within BPHYT_RS34095 at 3247.440 kb on + strand, within BPHYT_RS34095 at 3247.501 kb on - strand, within BPHYT_RS34095 at 3247.570 kb on - strand, within BPHYT_RS34095 at 3247.762 kb on - strand, within BPHYT_RS34095 at 3247.762 kb on - strand, within BPHYT_RS34095
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO1 MOI 10 remove 3,244,852 + +0.7 3,244,902 + +0.4 3,244,962 + +0.6 3,244,963 - +0.1 3,244,974 - +0.7 3,245,019 + +0.9 3,245,019 + +0.9 3,245,079 - BPHYT_RS34085 0.14 +0.6 3,245,117 + BPHYT_RS34085 0.18 -1.4 3,245,375 + BPHYT_RS34085 0.49 -1.1 3,245,771 + -0.1 3,245,772 - -0.5 3,245,866 + -0.3 3,245,867 - -0.1 3,245,956 + BPHYT_RS34090 0.11 +0.2 3,246,005 - BPHYT_RS34090 0.14 -1.6 3,246,005 - BPHYT_RS34090 0.14 +1.3 3,246,073 + BPHYT_RS34090 0.20 -0.1 3,246,074 - BPHYT_RS34090 0.20 +0.5 3,246,074 - BPHYT_RS34090 0.20 +0.4 3,246,158 + BPHYT_RS34090 0.26 -0.6 3,246,159 - BPHYT_RS34090 0.27 -0.6 3,246,160 + BPHYT_RS34090 0.27 +0.8 3,246,160 + BPHYT_RS34090 0.27 -0.5 3,246,161 - BPHYT_RS34090 0.27 -1.9 3,246,161 - BPHYT_RS34090 0.27 -0.2 3,246,487 + BPHYT_RS34090 0.52 -0.6 3,246,488 - BPHYT_RS34090 0.52 -1.5 3,246,885 - BPHYT_RS34090 0.83 -0.5 3,247,168 - -0.2 3,247,401 + BPHYT_RS34095 0.18 -1.3 3,247,440 + BPHYT_RS34095 0.21 -1.4 3,247,501 - BPHYT_RS34095 0.27 -0.6 3,247,570 - BPHYT_RS34095 0.34 -0.3 3,247,762 - BPHYT_RS34095 0.52 +1.1 3,247,762 - BPHYT_RS34095 0.52 -0.0
Or see this region's nucleotide sequence